文献类型：期刊文献

中文题名：氮离子注入纤维素酶产生菌的诱变效应研究

英文题名：Mutagenic Effects of Cellulase-Producing Strain by N^+ Implantation

作者：张宁[1] 蒋剑春[1]

第一作者：张宁

通信作者：Zhang, N.

机构：[1]中国林业科学研究院林产化学工业研究所,国家林业局林产化学工程重点开放性实验室,江苏南京210042

年份：2011

卷号：19

期号：6

起止页码：900-906

中文期刊名：应用基础与工程科学学报

外文期刊名：Journal of Basic Science and Engineering

收录：CSTPCD;;EI(收录号：20114914584661);Scopus(收录号：2-s2.0-82755190809);CSCD:【CSCD_E2011_2012】；

基金：国家林业局公益性行业专项(201004001)

语种：中文

中文关键词：氮离子注入;里氏木霉;总抗氧化能力;“双马鞍型”曲线

外文关键词：N ^＋ implantation ; Trichoderma reesei ; total antioxidase ; ＂double saddle shape＂ curve

分类号：TQ922.9;Q933

摘要：作为一种新型的生物诱变手段,离子注入技术以其独特的诱变机理和显著的生物学效应受到关注,被广泛地应用于植物、微生物育种和转基因.本文应用低能离子注入技术对纤维素酶产生菌里氏木霉(Trichoderma reesei)进行诱变选育,研究低能N+注入对其存活率、菌体总抗氧化能力(T-AOC)、蛋白质含量以及产酶能力的影响.结果表明,低能N+注入对T.reesei的诱变效应显著,T.reesei经N+离子注入后存活率曲线呈现"双马鞍型",而菌体注量-总抗氧化能力曲线与注量-存活率曲线呈现一致的变化趋势.由此推测,离子注入微生物所诱导的总抗氧化能力的强弱变化很有可能决定微生物的存活情况.在注入剂量为250×1014N+/cm2的条件下得到突变幅度提高最多的菌株,CMCA活力较对照增加了24.3%.
As a new means of biological mutation,ion implantation technology received much attention for its unique mutation mechanism and significant biological effects. It was widely used in plants, microorganisms breeding and transgenosis. In this paper, the mutation breeding on Tr/choderma reesei producing cellulase was studied after low energy ion beam implantation. The effects of lowenergy N ^＋ implantation on the survival rate, total antioxidase （T-AOC）, protein concentration and cellulose yield were investigated. The results showed that the mutation effect course by N ^＋ was remarkable. The dose-survival rate curve and dose-TOC curve all take ＂double saddle shape＂ with increasing N^＋ implantation doses. It can be considered that the changes of T-AOC activities probably decided the microbe survival situation after being implanted by low-energy N^＋. The high yielding mutation strain was obtained by screening at the doses of 250 × 10^14N^＋/cm^2, CMC enzyme activity increased by 24.3% compared with the control.