文献类型：期刊文献

英文题名：Robust CRISPR/Cas9 mediated gene editing of JrWOX11 manipulated adventitious rooting and vegetative growth in a nut tree species of walnut

作者：Chang, Yingying[1] Song, Xiaobo[1] Zhang, Qixiang[2] Zhang, Pu[1] Lei, Xiaoshuo[1] Pei, Dong[1]

第一作者：Chang, Yingying

通信作者：Pei, D[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Zhejiang A&F Univ, Sch Forestry & Biotechnol, Nurturing Stn, State Key Lab Subtrop Silviculture, Linan 311300, Peoples R China

年份：2022

卷号：303

外文期刊名：SCIENTIA HORTICULTURAE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000808366100002)】；

基金：Acknowledgments This research was supported by the Fundamental Research Funds of Chinese Academy of Forestry (CAFYBB2017ZA004-2) , the National Natural Science Foundation of China (No. 32101479) , and the State Key Laboratory of Tree Genetics and Breeding support program (Grant No. CAFYBB2019ZY001) . The author would like to thank Professor Caixia Gao (Chinese Academy of Sciences) for providing the plasmids for JrWOX11 edting.

语种：英文

外文关键词：Walnut; Genome-editing; CRISPR; Cas9; JrWOX11; Adventitious root

摘要：CRISPR/Cas9, a powerful genome-editing tool, has been widely adopted in model organisms but its application in walnut (Juglans regia L.), an economically important nut tree species grown worldwide, has been limited to date. To develop capacity for CRISPR/Cas9 technology in walnut, multiplexing CRISPR/Cas9 vectors expressing three single-guide RNAs (sgRNAs) targeting the walnut WUSCHEL-related homeobox 11 (WOX11) gene were tested in a model somatic embryo (SE) using Agrobacterium-mediated genetic transformation. Most resulting mutations were small indels, but large fragment deletions of 26 nt were also generated. Knockout of the JrWOX11 gene resulted in reduced adventitious root (AR) formation and vegetative growth. The results demonstrate successful site-directed mutagenesis in walnut with CRISPR/Cas9 and the usefulness of a model SE culture to validate genome editing systems. This study sheds light on the promising application of genome editing tools for boosting basic and translational research in this vital nut tree species.