文献类型：期刊文献

英文题名：Identification and Defensive Characterization of PmCYP720B11v2 from Pinus massoniana

作者：Liu, Bin[1,2] Xie, Yini[1,2] Yin, Huanhuan[3] Zhou, Zhichun[1,2] Liu, Qinghua[1,2]

第一作者：Liu, Bin

通信作者：Zhou, ZC[1];Liu, QH[1];Zhou, ZC[2];Liu, QH[2]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou 311400, Peoples R China;[2]Zhejiang Prov Key Lab Tree Breeding, Hangzhou 311400, Peoples R China;[3]Zhengzhou Bot Garden, Zhengzhou 450007, Peoples R China

年份：2022

卷号：23

期号：12

外文期刊名：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000817531700001)】；

基金：This work was supported by the Zhejiang Science and Technology Program(2020C02007), the Zhejiang Science and TechnologyMajor Program on Agricultural New Variety Breeding (2021C02070-5-2), and the National Natural Science Foundation of China (31470670).

语种：英文

外文关键词：Pinus massoniana; pine wood nematode; tissue-specific expression; defense response; drought stress tolerance

摘要：Pinus massoniana is a pioneer species for afforestation timber and oleoresin, while epidemics of pinewood nematode (PWN; Bursaphelenchus xylophilus) are causing a serious biotic disaster for P. massoniana in China. Importantly, resistant P. massoniana could leak copious oleoresin terpenoids to build particular defense fronts for survival when attacked by PWN. However, the defense mechanisms regulating this process remain unknown. Here, PmCYP720B11v2, a cytochrome P450 monooxygenase gene, was first identified and functionally characterized from resistant P. massoniana following PWN inoculation. The tissue-specific expression pattern and localization of PmCYP720B11v2 at the transcript and protein levels in resistant P. massoniana indicated that its upregulation in the stem supported its involvement in the metabolic processes of diterpene biosynthesis as a positive part of the defense against PWN attack. Furthermore, overexpression of PmCYP720B11v2 may enhance the growth and development of plants. In addition, PmCYP720B11v2 activated the metabolic flux of antioxidases and stress-responsive proteins under drought conditions and improved drought stress tolerance. Our results provide new insights into the favorable role of PmCYP720B11v2 in diterpene defense mechanisms in response to PWN attack in resistant P. massoniana and provide a novel metabolic engineering scenario to reform the stress tolerance potential of tobacco.