文献类型：期刊文献

英文题名：Identification of novel miRNAs and miRNA expression profiling in embryogenic tissues of Picea balfouriana treated by 6-benzylaminopurine

作者：Li, Qingfen[1,2] Deng, Cheng[1] Xia, Yan[2] Kong, Lisheng[3] Zhang, Hanguo[4] Zhang, Shougong[2] Wang, Junhui[2]

第一作者：Li, Qingfen

通信作者：Wang, JH[1]

机构：[1]South China Agr Univ, Coll Forestry & Landscape Architecture, Guangdong Key Lab Innovat Dev & Utilizat Forest P, State Key Lab Conservat & Utilizat Subtrop Agrobi, Guangzhou, Guangdong, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat,State Forestry A, Beijing, Peoples R China;[3]Univ Victoria, Dept Biol, Ctr Forest Biol, Victoria, BC, Canada;[4]Northeast Forestry Univ, State Key Lab Tree Genet & Breeding, Harbin, Peoples R China

年份：2017

卷号：12

期号：5

外文期刊名：PLOS ONE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000401314000008)】；

基金：Financial support for this study was provided by the Fundamental Research Funds for the Central Non-profit Research Institution of CAF, China (TGB2013011).

语种：英文

摘要：Here, we compared miRNA expression profiles in embryonic cell cultures of the conifer Picea balfouriana following application of the synthetic cytokinin 6-benzylaminopurine (6-BAP). We used next-generation sequencing to analyze three libraries of small RNAs from the treated embryogenic cell cultures and generated 24,000,000 raw reads from each of the libraries. Over 70 differentially regulated micro RNA (miRNA) families (>= 2 fold change in expression) were identified between pairs of treatments. A quantitative analysis showed that miR3633 and miR1026 were upregulated in tissues with the highest embryogenic ability. These two miRNAs were predicted to target genes encoding receptor-like protein kinase and GAMYB transcription factors, respectively. In one library, miR1160, miR5638, miR1315, and miR5225 were downregulated. These four miRNAs were predicted to target genes encoding APETALA2, calmodulin-binding protein, and calciumdependent protein kinase transcription factors. The expression patterns of the miRNAs and their targets were negatively correlated. Approximately 181 potentially novel P. balfouriana miRNAs were predicted from the three libraries, and seven were validated during the quantitative analysis. This study is the first report of differential miRNA regulation in tissues treated with 6-BAP during somatic embryogenesis. The differentially expressed miRNAs will be of value for investigating the mechanisms of embryogenic processes that are responsive to 6-BAP in P. balfouriana.