文献类型：期刊文献

英文题名：LkARF7 and LkARF19 overexpression promote adventitious root formation in a heterologous poplar model by positively regulating LkBBM1

作者：Tao, Gui-Yun[1] Xie, Yun-Hui[1] Li, Wan-Feng[1] Li, Kui-Peng[2] Sun, Chao[1] Wang, Hong-Ming[3] Sun, Xiao-Mei[1]

第一作者：Tao, Gui-Yun

通信作者：Sun, XM[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat,State Forestry &, Beijing 100091, Peoples R China;[2]Guangxi Forestry Res Inst, Liuzhou 530009, Guangxi, Peoples R China;[3]Tianshui Normal Univ, Coll Bioengn & Biotechnol, Tianshui 741000, Gansu, Peoples R China

年份：2023

卷号：6

期号：1

外文期刊名：COMMUNICATIONS BIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000963732500001)】；

基金：This work were supported by the Forestry Industry Research Special Funds for Public Welfare Projects (201504104)~and~the Special Funds of Research Institute of Forestry (LYSZX202002).

语种：英文

摘要：Overexpression of the transcription factors LkARF7 and LkARF19 promote adventitious root (AR) formation in a heterologous poplar model by positively regulating LkBBM1, providing further insight into the mechanisms of AR development. Cuttage propagation involves adventitious root formation induced by auxin. In our previous study, Larix kaempferi BABY BOOM 1 (LkBBM1), which is known to regulate adventitious root formation, was affected by auxin. However, the relationship between LkBBM1 and auxin remains unclear. Auxin response factors (ARFs) are a class of important transcription factors in the auxin signaling pathway and modulate the expression of early auxin-responsive genes by binding to auxin response elements. In the present study, we identified 14 L. kaempferi ARFs (LkARFs), and found LkARF7 and LkARF19 bound to LkBBM1 promoter and enhanced its transcription using yeast one-hybrid, ChIP-qPCR, and dual-luciferase assays. In addition, the treatment with naphthalene acetic acid promoted the expression of LkARF7 and LkARF19. We also found that overexpression of these two genes in poplar promoted adventitious root formation. Furthermore, LkARF19 interacted with the DEAD-box ATP-dependent RNA helicase 53-like protein to form a heterodimer to regulate adventitious root formation. Altogether, our results reveal an additional regulatory mechanism underlying the control of adventitious root formation by auxin.