文献类型：期刊文献

英文题名：PsGSTF3, an Anthocyanin-Related Glutathione S-Transferase Gene, Is Essential for Petal Coloration in Tree Peony

作者：Han, Lulu[1] Zhou, Lin[1] Zou, Hongzhu[1] Yuan, Meng[1] Wang, Yan[1]

第一作者：Han, Lulu

通信作者：Wang, Y[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, Key Lab Tree Breeding & Cultivat Natl Forestry &, Beijing 100091, Peoples R China

年份：2022

卷号：23

期号：3

外文期刊名：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000759355600001)】；

基金：Funding: This research was funded by grants from the National Key Research and Development Program (Grant No. 2018YFD1000405), and the National Natural Science Foundation of China (Grant No. 31972456).

语种：英文

外文关键词：Paeonia suffruticosa; anthocyanin; transport; glutathione S-transferase

摘要：Anthocyanins, as the most important chromogenic substances in flavonoids, are responsible for the red, purple, and blue coloration of flowers. Anthocyanins are synthesized in the cytoplasmic surface of the endoplasmic reticulum (ER) but accumulate predominantly in the vacuole, while glutathione S-transferases (GSTs) are considered to be mainly responsible for the transport process. Our previous studies showed that the expression of PsGSTF3 was positively correlated with anthocyanin content in tree peony tissues, which is a key candidate gene for anthocyanin accumulation. Here, we successfully cloned and characterized full-length PsGSTF3 containing three exons and two introns. Subcellular localization showed that PsGSTF3 was localized in the nucleus and ER membrane. Functional complementation of the Arabidopsis transparent testa19 (tt19) mutant indicated that PsGSTF3 was responsible for the transport of anthocyanins but not of proanthocyanidins (PAs). Virus-induced gene silencing (VIGS) of PsGSTF3 not only led to a decrease in anthocyanin accumulation but also caused a reduction of structural genes in the anthocyanin biosynthesis pathway (ABP) to varying degrees. Heterologous overexpression of PsGSTF3 was found to increase the anthocyanin accumulation in tobacco petals. Furthermore, the yeast two-hybrid (Y2H) assay showed that PsGSTF3 interacted with PsDFR, which together contributed to the coloration of petals. In conclusion, these results demonstrate that PsGSTF3 encodes an important GST transporter of anthocyanin in tree peony petals and provides a new perspective for the associated transport and regulatory mechanisms.