文献类型：期刊文献

英文题名：Next-generation sequencing-based mRNA and microRNA expression profiling analysis revealed pathways involved in the rapid growth of developing culms in Moso bamboo

作者：He, Cai-yun[1,2] Cui, Kai[1,2,3] Zhang, Jian-guo[1,2] Duan, Ai-guo[1,2] Zeng, Yan-fei[1,2]

第一作者：何彩云

通信作者：Zhang, JG[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, State Forestry Adm, Key Lab Tree Breeding & Cultivat, Beijing 100091, Peoples R China;[3]Chinese Acad Forestry, Res Inst Resources Insects, Kunming 650224, Peoples R China

年份：2013

卷号：13

期号：1

外文期刊名：BMC PLANT BIOLOGY

收录：;Scopus(收录号：2-s2.0-84882310601);WOS:【SCI-EXPANDED(收录号:WOS:000323751800001)】；

基金：We acknowledge the Beijing Genomics Institute at Shenzhen for its assistance in original data processing and related bioinformatics analysis. We would like to thank Zhong-fu Deng and Jian-jiu Liu for their help in sample collection. This work was supported by grants from the National Natural Science Foundation of China (30972332 and 31100454).

语种：英文

摘要：Background: As one of the fastest-growing lignocellulose-abundant plants on Earth, bamboos can reach their final height quickly due to the expansion of individual internodes already present in the buds; however, the molecular processes underlying this phenomenon remain unclear. Moso bamboo (Phyllostachys heterocycla cv. Pubescens) internodes from four different developmental stages and three different internodes within the same stage were used in our study to investigate the molecular processes at the transcriptome and post-transcriptome level. Results: Our anatomical observations indicated the development of culms was dominated by cell division in the initial stages and by cell elongation in the middle and late stages. The four major endogenous hormones appeared to actively promote culm development. Using next-generation sequencing-based RNA-Seq, mRNA and microRNA expression profiling technology, we produced a transcriptome and post-transcriptome in possession of a large fraction of annotated Moso bamboo genes, and provided a molecular basis underlying the phenomenon of sequentially elongated internodes from the base to the top. Several key pathways such as environmental adaptation, signal transduction, translation, transport and many metabolisms were identified as involved in the rapid elongation of bamboo culms. Conclusions: This is the first report on the temporal and spatial transcriptome and gene expression and microRNA profiling in a developing bamboo culms. In addition to gaining more insight into the unique growth characteristics of bamboo, we provide a good case study to analyze gene, microRNA expression and profiling of non-model plant species using high-throughput short-read sequencing. Also, we demonstrate that the integrated analysis of our multi-omics data, including transcriptome, post-transcriptome, proteome, yield more complete representations and additional biological insights, especially the complex dynamic processes occurring in Moso bamboo culms.