文献类型：期刊文献

英文题名：Genetic diversity and population structure of Taxus cuspidata in the Changbai Mountains assessed by chloroplast DNA sequences and microsatellite markers

作者：Cheng, Bei-bei[1] Zheng, Yong-qi[1] Sun, Qi-wu[1]

第一作者：Cheng, Bei-bei

通信作者：Sun, QW[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, Lab Mol Identificat Plant Varieties, Key Lab Silviculture State Forestry Adm,State Key, Beijing 100091, Peoples R China

年份：2015

卷号：63

起止页码：157-164

外文期刊名：BIOCHEMICAL SYSTEMATICS AND ECOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000367485900024)】；

基金：The present study was supported by the project (2130211) of Rescue and breeding of rare and endangered wild plants (Taxus). We thank Shao-shuai Yu of the Chinese Academy of Forestry, National Nature Preserve of Wangqing County, Forestry Department of Liaoning Province and Forestry Department of Heilongjiang Province for sample collection.

语种：英文

外文关键词：Taxus cuspidata; Changbai Mountains; Genetic diversity; Population structure; Geographic pattern

摘要：Taxus cuspidata, a tree species with high economic value because of its anticancer properties, is experiencing severe reduction in populations across its range in China. We examined one chloroplast DNA (cpDNA) region (petA-psbE) and 9 nuclear simple sequence repeats (SSRs) loci variations among seven populations in the Changbai Mountains of China to investigate the levels of genetic diversity and population structure. A moderate level of haplotype diversity (H-T = 0.625), low nuclear microsatellite diversity (H-E = 0.261 +/- 0.028), significant genetic differences (F-ST = 0.065) and substantial gene flow (Nm = 2.806) were observed. Most of the total genetic variation was partitioned within the population (87.8% and 94.0% for cpDNA and SSRs, respectively). Our haplotype identification permutation tests revealed that G(ST) > N-ST, indicating an absence of phylogeographic structure in T. cuspidata. Neither STRUCTURE nor UPGMA analyses showed any geographic pattern in T. cuspidata populations. By comparatively analyzing the genetic diversity and survival situation of T. cuspidata, our results provide a theoretical foundation for the resource protection, utilization cultivation and breeding of this valuable plant. (C) 2015 Elsevier Ltd. All rights reserved.