文献类型：期刊文献

英文题名：Evidence for Biotrophic Lifestyle and Biocontrol Potential of Dark Septate Endophyte Harpophora oryzae to Rice Blast Disease

作者：Su, Zhen-Zhu[1] Mao, Li-Juan[2] Li, Na[1] Feng, Xiao-Xiao[1] Yuan, Zhi-Lin[1,3] Wang, Li-Wei[1,4] Lin, Fu-Cheng[1,5] Zhang, Chu-Long[1]

第一作者：Su, Zhen-Zhu

通信作者：Zhang, CL[1]

机构：[1]Zhejiang Univ, Inst Biotechnol, State Key Lab Rice Biol, Hangzhou 310003, Zhejiang, Peoples R China;[2]Zhejiang Univ, Anal Ctr Agrobiol & Environm Sci, Hangzhou 310003, Zhejiang, Peoples R China;[3]Chinese Acad Forestry, Inst Subtrop Forestry, Fuyang, Zhejiang, Peoples R China;[4]Hangzhou Normal Univ, Sch Hlth Management, Dept Pharmaceut Sci, Hangzhou, Zhejiang, Peoples R China;[5]Zhengzhou Tobacco Res Inst CNTC, China Tobacco Gene Res Ctr, Zhenzhou, Peoples R China

年份：2013

卷号：8

期号：4

外文期刊名：PLOS ONE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000317908700025)】；

基金：This work was financially supported by the National Natural Science Foundation of China (30970097 and 30925025) (http://www.nsfc.gov.cn/Portal0/default152.htm), and the Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT0943) of Ministry of Education of the People's Republic of China (http://www.moe.gov.cn/). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

语种：英文

摘要：The mutualism pattern of the dark septate endophyte (DSE) Harpophora oryzae in rice roots and its biocontrol potential in rice blast disease caused by Magnaporthe oryzae were investigated. Fluorescent protein-expressing H. oryzae was used to monitor the colonization pattern. Hyphae invaded from the epidermis to the inner cortex, but not into the root stele. Fungal colonization increased with root tissue maturation, showing no colonization in the meristematic zone, slight colonization in the elongation zone, and heavy colonization in the differentiation zone. H. oryzae adopted a biotrophic lifestyle in roots accompanied by programmed cell death. Real-time PCR facilitated the accurate quantification of fungal growth and the respective plant response. The biocontrol potential of H. oryzae was visualized by inoculation with eGFP-tagged M. oryzae in rice. H. oryzae protected rice from M. oryzae root invasion by the accumulation of H2O2 and elevated antioxidative capacity. H. oryzae also induced systemic resistance against rice blast. This systemic resistance was mediated by the OsWRKY45-dependent salicylic acid (SA) signaling pathway, as indicated by the strongly upregulated expression of OsWRKY45. The colonization pattern of H. oryzae was consistent with the typical characteristics of DSEs. H. oryzae enhanced local resistance by reactive oxygen species (ROS) and high antioxidative level and induced OsWRKY45-dependent SA-mediated systemic resistance against rice blast.