文献类型：期刊文献

中文题名：红树林溶磷菌的初步鉴定、溶磷能力测定及其优化培养

英文题名：Primary Identification, Capability of Phosphate-Solubilization and Optimization of Medium of Some Microorganism from Mangrove

作者：陈俊[1,2] 陆俊锟[1] 康丽华[1] 杨振德[2]

第一作者：陈俊

机构：[1]中国林业科学研究院热带林业研究所;[2]广西大学林学院

年份：2009

期号：8

起止页码：1183-1188

中文期刊名：微生物学通报

外文期刊名：Microbiology

收录：CSTPCD;;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金：国家自然科学基金项目(No.30671668)

语种：中文

中文关键词：红树林;溶磷菌;溶磷能力;优化培养

外文关键词：Mangrove, Phosphate-solubilizing microorganism, Phosphate solubilization capacity, Optimized culture

分类号：S144.1;TQ921.7

摘要：对分离来自华南红树林的溶磷菌进行16SrDNA或ITS等基因水平上的初步鉴定,测定其溶解无机磷的能力,并对溶磷菌的生长培养基条件进行优化。结果表明,溶磷真菌的溶磷效果明显强于溶磷细菌,且溶磷真菌的溶解无机磷能力与培养液的pH呈极显著负相关,而溶磷细菌的溶磷能力与pH没显著相关关系。单因素实验表明,对供试菌株生长的合适碳源为麦芽糖,氮源为尿素。通过正交实验得到的优化培养基为麦芽糖5g/L、尿素0.05g/L、NaCl5g/L、pH5,在30°C下培养48h菌落总数可达6.06×109CFU/mL。
The genetic indentification of 16S rDNA or ITS, capability of phosphate-solubilization and pH of medium, and optimization of medium of some microorganism isolated from mangrove were investigated in this study. The result showed that the fungi normally had much higher capacity to dissolve the inorganic phosphate than the bacteria, the capacity of the fungi was closely correlated to the pH of medium, but the relationship was weak for the bacteria. It was illustrated by single factor experiments that the optimal carbon and nitrogen sources were maltose and urea respectively. The orthogonal design was employed in testing the optimum composition of medium composed of 5 g/L maltose, 0.05 g/L urea, 5 g/L NaCl, pH 5. In this optimal medium, the effectively enrichment of bacteria could reach up to 6.06× 10^9 CFU/mL under 30℃ for 48 hours cultivation.