文献类型：期刊文献

中文题名：土壤调理剂对科尔沁盐渍化草原土壤细菌群落多样性的影响

英文题名：Impact of Soil Conditioners on Soil Bacterial Community Diversity in Salinized Grasslands of the Horqin Region

作者：石涵宇[1,2,3] 姚洪隽锡[4] 付天生[5] 杨柳[1,2,3] 马星宇[1,2,3] 付桂红[6] 姚斌[1,2,3]

第一作者：石涵宇

机构：[1]中国林业科学研究院生态保护与修复研究所,北京100091;[2]中国林业科学研究院荒漠化研究所,北京100091;[3]甘肃敦煌-库姆塔格荒漠生态系统定位观测研究站,敦煌736200;[4]贵州大学农学院,贵阳550025;[5]扎鲁特旗草源农牧业投资发展集团有限公司,通辽029100;[6]扎鲁特旗鲁北镇综合保障和技术推广中心,通辽029100

年份：2025

卷号：5

期号：3

起止页码：26-34

中文期刊名：陆地生态系统与保护学报

外文期刊名：Terrestrial Ecosystem and Conservation

基金：“十四五”国家重点研发计划专题(2023YFF1304103-04);中国林业科学研究院生态保护与修复研究所科技项目(STSTC2023004)。

语种：中文

中文关键词：盐渍化草原;细菌;科尔沁;土壤调理剂;群落多样性

外文关键词：saline-alkaline grassland;bacteria;Horqin;soil amendments;community diversity

分类号：S154.3;S156.4

摘要：【目的】探究不同调理剂对盐渍化草原土壤性质和细菌群落结构的影响,从土壤微生物角度为改良盐渍化草原提供理论依据。【方法】以内蒙古科尔沁盐渍化草原土壤为对象,采用高通量测序技术,以健康草原(JK)土壤作为理想修复参照,探讨了盐渍化草原土壤不添加外源调理剂(CK)和外源添加脱硫石膏(DG)、过磷酸钙(SS)、腐殖酸(HA)和生物质炭(BC)对土壤化学性质及细菌群落多样性与结构组成的影响。【结果】相较于CK,DG显著降低了科尔沁盐渍化草原土壤pH值;HA、BC处理显著增加了盐渍化草原土壤有机质含量;SS处理显著提高了土壤全磷含量。外源添加调理剂改变了土壤细菌群落组成,但细菌多样性改变不显著。放线菌门、变形菌门和酸杆菌门为主要优势类群。相较于CK,DG、SS、HA和BC处理皆促进了放线菌门和厚壁菌门的富集,同时降低了酸杆菌门和拟杆菌门相对丰度。与JK相比,盐渍化草原土壤的拟杆菌门、芽单胞菌门和黏菌门占比增加。土壤有机质或有机碳、全氮含量及pH值和电导率是主导细菌群落结构变化的关键环境因子。【结论】添加土壤调理剂能在一定程度上改变盐渍化草原土壤微生物的群落组成,提升土壤质量,研究结果为科尔沁盐渍化草原土壤修复及养分提升提供了科学参考。
【Objective】This study investigates the effects of various soil amendments on the physicochemical properties and bacterial community structure of salinized-alkalized steppe soils,aiming to provide a microbial perspective for the ecological restoration of degraded steppe ecosystems.【Method】Salinized-alkalized soils from the Horqin Sandy Land steppe in Inner Mongolia were used as the study system.High-throughput sequencing was employed to evaluate the impacts of four exogenous soil amendments—desulfurized gypsum(DG),superphosphate(SS),humic acid(HA),and biochar(BC)—on soil physicochemical characteristics and bacterial community diversity,composition,and structure.A healthy steppe soil(JK)was used as a reference to represent ideal restoration conditions.【Result】Compared with the control(CK),DG has significantly reduced soil pH;HA and BC significantly increased soil organic matter(SOM)content;SS treatment significantly enhanced total phosphorus(TP)levels.While the amendments altered bacterial community composition,they did not significantly affect overall bacterial diversity.Actinobacteriota,Proteobacteria,and Acidobacteriota were the three most dominant bacterial phyla.Compared with CK,treatments of DG,SS,HA,and BC have increased the relative abundance of Actinobacteriota and Firmicutes,while decreased the abundance of Acidobacteriota and Bacteroidota.In comparison to JK,salinized-alkaline soils showed higher proportions of Bacteroidota,Gemmatimonadota,and Myxococcota.Soil organic matter(SOM),soil organic carbon(SOC),total nitrogen(TN),pH-value,and electrical conductivity(EC)were identified as the main environmental factors shaping bacterial community structure.【Conclusion】The addition of soil amendments can,to some extent,alter the microbial community composition of saline-alkali grassland soils and improve soil quality.The findings of this study provide a scientific reference for soil restoration and nutrient enhancement in the saline-alkali grasslands of the Horqin region.