文献类型：期刊文献

中文题名：应用DGGE技术分析青藏铁路沿线的土壤细菌种群多样性

英文题名：Population diversity of soil bacteria along the Qinghai-Tibet railway by DGGE analysis

作者：李潞滨[1] 刘振静[1] 庄彩云[1] 周金星[1] 杨凯[2] 韩继刚[3]

第一作者：李潞滨

机构：[1]中国林业科学研究院林业研究所国家林业局林木培育重点实验室,北京100091;[2]北京农学院农业应用新技术北京市重点实验室,北京102206;[3]河北大学生命科学学院,河北保定071002

年份：2008

卷号：27

期号：5

起止页码：751-755

中文期刊名：生态学杂志

外文期刊名：Chinese Journal of Ecology

收录：CSTPCD;;Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：铁道部重大科技计划资助项目(2004G009)

语种：中文

中文关键词：青藏铁路;细菌;种群多样性;植被类型

外文关键词：Qinghai-Tibet railway ; bacteria ; population diversity ; vegetation type

分类号：Q939.1

摘要：选择青藏铁路沿线不同海拔高度的10个地点采集土壤样品,直接提取样品中的总DNA,以巢式PCR扩增细菌16S rDNA片段,应用变性梯度凝胶电泳(DGGE)技术分离PCR扩增的16S rDNA片段,研究土壤细菌的种群多样性。结果表明,青藏铁路沿线高海拔地区具有较为丰富的细菌种群多样性,植被类型是影响青藏铁路沿线土壤细菌种群多样性的重要因素,也是影响土壤细菌种群结构相似性的重要因素,而海拔高度等是次要的影响因素;具有相似植被类型的土壤样品,其细菌种群多样性随海拔的升高而下降。
Soil samples were collected from ten different-altitude sites along the Qinghai-Tibet railway, and the community DNA was extracted from the soil samples directly. Bacterial 16S rRNA gene fragment was amplified by nested PCR from the community DNA. Soil bacterial population diversity was analyzed by 16S rDNA-PCR-DGGE. The results showed that the bacterial population diversity was abundant in this high altitude area. Vegetation type was an important factor influencing the bacterial diversity and population similarity in this area, and the altitude acted as a minor impact factor. The bacterial population diversity of soil samples with similar vegetation type decreased with the increasing altitude.