文献类型：期刊文献

中文题名：多花黄精根茎芽高效组培增殖和生根体系研究

英文题名：An efficient proliferation and inducing roots system of tissue culture in Polygonatum cyrtonema

作者：周新华[1] 厉月桥[1] 王丽云[1] 肖智勇[2] 钟文斌[1] 陈传松[1] 邓煌蔚[1] 黄维荣[1]

第一作者：周新华

机构：[1]中国林业科学研究院亚热带林业实验中心;[2]宜春市林业科学研究所

年份：2016

卷号：34

期号：1

起止页码：51-56

中文期刊名：经济林研究

外文期刊名：Nonwood Forest Research

收录：CSTPCD;;北大核心:【北大核心2014】；

基金：中央级公益性科研院所基本科研业务费专项资金项目(CAFYBB2014MB007)

语种：中文

中文关键词：多花黄精;组织培养;增殖;生根;工厂化育苗

外文关键词：Polygonatum cyrtonema; tissue culture; proliferation; inducing roots; industrialized breeding

分类号：S604.3

摘要：为了建立多花黄精高效组培增殖和生根体系,给其工厂化、规模化育苗提供可行的关键技术方案,以其根茎芽培养的无菌苗为研究对象,就外植体的不同采集时间和切分处理方式、增殖培养周期、继代培养次数和培养基中活性炭的浓度对其根茎芽增殖和生根的影响情况进行了离体组织培养试验。结果表明：3~4月采集的外植体其生长势和腋芽萌发的表现均最好,平均萌发腋芽6.6个;外植体的最佳切分方式为处理Ⅰ,即将根茎芽切成1份的无菌块茎;最佳增殖培养周期为45 d,平均萌发芽数为6.5个;最适继代培养次数为7次,继代培养7次后平均每个根茎能诱导6.2个不定芽,能诱导的平均根数达20.8条;增殖培养基中活性炭的最佳浓度为0.05 g·L-1,生根培养基中活性炭的最佳浓度为0.20 g·L-1。
To build a of efficient proliferation and inducing roots system of tissue culture in Polygon atum cyrtonema, and to provide some theoretical support for scale and industrialized breeding, taking aseptic seedlings cultured from rhizome buds as research objects, effects of some factors on bud proliferation and inducing roots were researched in the tissue culture experiments in vitro, including different sampling time, segmentation app roaches, proliferation culture period, subculture times, and activated carbon concentrations in media. The results showed that growth status and axillary bud germination status of the explants sampled in March and April was best, and mean number of germinated axillary buds was 6.6. One explants per bud was the best segmentation way. Forty-five days were the best proliferation period, and mean number of germinated buds were 6.5. The optimum subculture times was seven, and then a rhizome explants could be induced 6.2 adventitious buds and 20.8 roots. In addition, the optimal concentration of activated carbon in proliferation medium 0.05 g·L- 1, and that in inducing root medium was 0.20 g·L- 1.