文献类型：期刊文献

英文题名：Integrated mRNA and miRNA transcriptome reveal a cross-talk between developing response and hormone signaling for the seed kernels of Siberian apricot

作者：Niu, Jun[1] Wang, Jia[1] An, Jiyong[1] Liu, Lili[1] Lin, Zixin[1] Wang, Rui[1] Wang, Libing[2] Ma, Chao[1] Shi, Lingling[1] Lin, Shanzhi[1]

第一作者：Niu, Jun

通信作者：Lin, SZ[1]

机构：[1]Beijing Forestry Univ, Key Lab Genet & Breeding Forest Trees & Ornamenta, Natl Engn Lab Tree Breeding, Coll Biol Sci & Biotechnol,Minist Educ, Beijing 10083, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, Beijing 10091, Peoples R China

年份：2016

卷号：6

外文期刊名：SCIENTIFIC REPORTS

收录：;Scopus(收录号：2-s2.0-84992426915);WOS:【SCI-EXPANDED(收录号:WOS:000386206800001)】；

基金：This research was supported by the National Natural Sciences Foundation of China (No. 31570653).

语种：英文

摘要：Recently, our transcriptomic analysis has identified some functional genes responsible for oil biosynthesis in developing SASK, yet miRNA-mediated regulation for SASK development and oil accumulation is poorly understood. Here, 3 representative periods of 10, 30 and 60 DAF were selected for sRNA sequencing based on the dynamic patterns of growth tendency and oil content of developing SASK. By miRNA transcriptomic analysis, we characterized 296 known and 44 novel miRNAs in developing SASK, among which 36 known and 6 novel miRNAs respond specifically to developing SASK. Importantly, we performed an integrated analysis of mRNA and miRNA transcriptome as well as qRT-PCR detection to identify some key miRNAs and their targets (miR156-SPL, miR160-ARF18, miR164-NAC1, miR171h-SCL6, miR172-AP2, miR395-AUX22B, miR530-P2C37, miR393h-TIR1/AFB2 and psi-miRn5-SnRK2A) potentially involved in developing response and hormone signaling of SASK. Our results provide new insights into the important regulatory function of cross-talk between development response and hormone signaling for SASK oil accumulation.