文献类型：期刊文献

英文题名：Plant biotechnology for lignocellulosic biofuel production

作者：Li, Quanzi[1,2] Song, Jian[3] Peng, Shaobing[4] Wang, Jack P.[1,5] Qu, Guan-Zheng[1] Sederoff, Ronald R.[5] Chiang, Vincent L.[1,5]

第一作者：Li, Quanzi;李全梓

通信作者：Li, QZ[1]

机构：[1]Northeast Forestry Univ, State Key Lab Tree Genet & Breeding, Harbin, Peoples R China;[2]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing, Peoples R China;[3]Dezhou Univ, Coll Life Sci, Dezhou, Shandong, Peoples R China;[4]Northwest A&F Univ, Coll Forestry, Yangling, Shaanxi, Peoples R China;[5]N Carolina State Univ, Dept Forestry & Environm Resources, Forest Biotechnol Grp, Raleigh, NC 27695 USA

年份：2014

卷号：12

期号：9

起止页码：1174-1192

外文期刊名：PLANT BIOTECHNOLOGY JOURNAL

收录：;Scopus(收录号：2-s2.0-84911961843);WOS:【SCI-EXPANDED(收录号:WOS:000345702500004)】；

基金：This work is supported by National Natural Science Foundation of China (Grant 31370593 to Q.L.), the Southeastern Sun Grant Initiative Competitive Grants Programs and the National Science Foundation of USA, Plant Genome Research Program (Grant DBI-0922391 to V.L.C.).

语种：英文

外文关键词：biofuel; biotechnology; cell wall; lignocellulose

摘要：Lignocelluloses from plant cell walls are attractive resources for sustainable biofuel production. However, conversion of lignocellulose to biofuel is more expensive than other current technologies, due to the costs of chemical pretreatment and enzyme hydrolysis for cell wall deconstruction. Recalcitrance of cell walls to deconstruction has been reduced in many plant species by modifying plant cell walls through biotechnology. These results have been achieved by reducing lignin content and altering its composition and structure. Reduction of recalcitrance has also been achieved by manipulating hemicellulose biosynthesis and by overexpression of bacterial enzymes in plants to disrupt linkages in the lignin-carbohydrate complexes. These modified plants often have improved saccharification yield and higher ethanol production. Cell wall-degrading (CWD) enzymes from bacteria and fungi have been expressed at high levels in plants to increase the efficiency of saccharification compared with exogenous addition of cellulolytic enzymes. In planta expression of heat-stable CWD enzymes from bacterial thermophiles has made autohydrolysis possible. Transgenic plants can be engineered to reduce recalcitrance without any yield penalty, indicating that successful cell wall modification can be achieved without impacting cell wall integrity or plant development. A more complete understanding of cell wall formation and structure should greatly improve lignocellulosic feedstocks and reduce the cost of biofuel production.