文献类型：期刊文献

英文题名：MicroRNA319-mediated gene regulatory network impacts leaf development and morphogenesis in poplar

作者：Cheng, Yanxia[1,2] Wang, Lihu[3] Abbas, Manzar[4] Huang, Xiong[2,5] Wang, Qiao[2,5] Wu, Aimin[6,7] Wei, Hairong[8] Peng, Shaobing[1] Dai, Xinren[2] Li, Quanzi[2,5]

第一作者：Cheng, Yanxia

通信作者：Peng, SB[1];Dai, XR[2]

机构：[1]Northwest A&F Univ, Coll Forestry, Yangling 712100, Shaanxi, Peoples R China;[2]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[3]Hebei Univ Engn, Coll Landscape & Ecol Engn, Handan 056038, Hebei, Peoples R China;[4]Yibin Univ, Sch Agr Forestry & Food Engn, Key Lab Aromat Plant Resources Exploitat & Utiliza, Yibin 644000, Sichuan, Peoples R China;[5]Chinese Acad Forestry, Res Inst Forestry, Beijing 100091, Peoples R China;[6]South China Agr Univ, State Key Lab Conservat & Utilizat Subtrop Agrobio, Guangzhou 510642, Guangdong, Peoples R China;[7]South China Agr Univ, Coll Forestry & Landscape Architecture, Guangdong Key Lab Innovat Dev & Utilizat Forest Pl, Guangzhou 510642, Guangdong, Peoples R China;[8]Michigan Technol Univ, Coll Forest Resources & Environm Sci, Houghton, MI 49931 USA

年份：2021

卷号：1

外文期刊名：FORESTRY RESEARCH

收录：Scopus(收录号：2-s2.0-85115883521);WOS:【ESCI(收录号:WOS:001385917400004)】；

基金：This work was supported by a grant from Fundamental Research Funds of CAF (CAFYBB2017ZY001) . We also thank Prof. Dayong Li of Beijing Academy of Agriculture and Forestry Sciences for sharing plasmid of MIR319a over-expression.

语种：英文

摘要：MicroRNA319 (miR319) has been implicated in leaf development in a number of plant species. Here we study the roles of miR319a and its regulated network in leaf development in poplars. Over-expression of miR319a in Populus alba x Populus glandulosa caused dwarf statures, narrow leaf blades and serrated leaf margins. The vascular bundles and bundle sheaths in transgenic leaves had more layers of cells than those in the leaves of control plants, indicating enhanced lignification in these cells. Among the 93 putative targets of miR319a predicted with the psRNATarget tool, only three genes, TCP ( TEOSINTEBRANCHED1 , CYCLOIDEA, and PROLIFERATING CELL NUCLEAR ANTIGEN BINDING FACTOR), were differentially expressed in the leaves of MIR319a-over-expression transgenic lines. With the RNA-seq data sets from multiple MIR319a over- expression transgenic lines, we built a three-layered gene regulatory network mediated by miR319a using Top-down graphic Gaussian model (GGM) algorithm that is capable of capturing causal relationships from transcriptomic data. The results support that miR319a primarily regulates the lignin biosynthesis, leaf development and differentiation as well as photosynthesis via miR319-MEE35/TCP4, miR319-TCP2 and miR319-TCP2-1 regulatory modules.