文献类型：期刊文献

英文题名：BeMADS1 is a key to delivery MADSs into nucleus in reproductive tissues-De novo characterization of Bambusa edulis transcriptome and study of MADS genes in bamboo floral development

作者：Shih, Ming-Che[1] Chou, Ming-Lun[2] Yue, Jin-Jun[3] Hsu, Cheng-Tran[1] Chang, Wan-Jung[1] Ko, Swee-Suak[1,4] Liao, De-Chih[1] Huang, Yao-Ting[5] Chen, Jeremy J. W.[6] Yuan, Jin-Ling[3] Gu, Xiao-Ping[3] Lin, Choun-Sea[1]

第一作者：Shih, Ming-Che

通信作者：Lin, CS[1]

机构：[1]Acad Sinica, Agr Biotechnol Res Ctr, Taipei 115, Taiwan;[2]Tzu Chi Univ, Dept Life Sci, Hualien, Taiwan;[3]Chinese Acad Forestry, Res Inst Subtrop Forestry, Fuyang, Peoples R China;[4]Acad Sinica, Biotechnol Ctr Southern Taiwan, Tainan, Taiwan;[5]Natl Chung Cheng Univ, Dept Comp Sci & Informat Engn, Chiayi, Taiwan;[6]Natl Chung Hsing Univ, Inst Biomed Sci, Taichung 40227, Taiwan

年份：2014

卷号：14

期号：1

外文期刊名：BMC PLANT BIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000338676100001)】；

基金：We thank Shu-Chen Shen from the Confocal Microscopic Core Facility at Academia Sinica for assistance in confocal microscopy images. We also think Hui-Ting Yang and Yi-Chen Lien for assistance with in situ hybridization. We would like to thank Ms. Anita K. Snyder for giving comments on the manuscript. This work was supported by the National Science Council, Taiwan (Grants 99-2313-B-001 -001 -MY3 and 102-2313-B-001 -002 -;CSL) and Presidential Foundation of the Research Institute of Subtropical Forestry, China (RISF2013004; JJY, JLY, and XPG).

语种：英文

外文关键词：Hybrid transcriptomics; Protein translocation; In vitro flowering; ABCDE model; In situ hybridization; Juvenility

摘要：Background: The bamboo Bambusa edulis has a long juvenile phase in situ, but can be induced to flower during in vitro tissue culture, providing a readily available source of material for studies on reproductive biology and flowering. In this report, in vitro-derived reproductive and vegetative materials of B. edulis were harvested and used to generate transcriptome databases by use of two sequencing platforms: Illumina and 454. Combination of the two datasets resulted in high transcriptome quality and increased length of the sequence reads. In plants, many MADS genes control flower development, and the ABCDE model has been developed to explain how the genes function together to create the different whorls within a flower. Results: As a case study, published floral development-related OsMADS proteins from rice were used to search the B. edulis transcriptome datasets, identifying 16 B. edulis MADS (BeMADS). The BeMADS gene expression levels were determined qRT-PCR and in situ hybridization. Most BeMADS genes were highly expressed in flowers, with the exception of BeMADS34. The expression patterns of these genes were most similar to the rice homologs, except BeMADS18 and BeMADS34, and were highly similar to the floral development ABCDE model in rice. Transient expression of MADS-GFP proteins showed that only BeMADS1 entered leaf nucleus. BeMADS18, BeMADS4, and BeMADS1 were located in the lemma nucleus. When co-transformed with BeMADS1, BeMADS15, 16, 13, 21, 6, and 7 translocated to nucleus in lemmas, indicating that BeMADS1 is a key factor for subcellular localization of other BeMADS. Conclusion: Our study provides abundant B. edulis transcriptome data and offers comprehensive sequence resources. The results, molecular materials and overall strategy reported here can be used for future gene identification and for further reproductive studies in the economically important crop of bamboo.