文献类型：期刊文献

中文题名：四数九里香种子自然萌发和离体萌发条件筛选

英文题名：Screening of natural germination and in vitro germination conditions of Murraya paniculata seeds

作者：蒋建林[1,2] 王森[1,2] 李畅[3] 郭红艳[4]

第一作者：蒋建林

机构：[1]中南林业科技大学经济林培育与保护教育部重点实验室;[2]中南林业科技大学经济林育种与栽培国家林业局重点实验室;[3]江西环境工程职业学院;[4]中国林业科学研究院亚热带林业实验中心

年份：2017

卷号：35

期号：3

起止页码：93-101

中文期刊名：经济林研究

外文期刊名：Nonwood Forest Research

收录：CSTPCD;;北大核心:【北大核心2014】；

基金：国家"十二五"农村领域科技计划课题研究任务合约(2013BAD14D0502)

语种：中文

中文关键词：四数九里香;植物组织培养;生长素;活性炭;褐化

外文关键词：Murraya paniculata; plant tissue culture; auxin; activated carbon; browning

分类号：S688

摘要：为提高四数九里香种子萌发率,研究了最佳浓硫酸处理种子的时间及不同激素处理对四数九里香种子自然萌发的影响,以完整种子和带胚子叶作为外植体,研究不同质量浓度的6-BA、NAA、IBA、GA3对外植体生长的影响,及不同质量浓度活性炭对外植体褐化的影响。结果表明,浓硫酸最佳处理时间为1 min;用200×10-6的IBA处理过的种子发芽率最高,达50%;其次是50×10^(-6)和150×10^(-6)的NAA,其萌发率均为45%;外植体在添加2.5 mg/L 6-BA、0.2 mg/L IBA、0.1 mg/L GA3或0.2 mg/L NAA的培养基上诱导率较高,活性炭为1.5 g/L时外植体褐化程度最低;四数九里香胚培养的最佳培养基为添加2.5 mg/L 6-BA、0.1 mg/L GA3、0.2mg/L NAA和1.5 g/L活性炭的MS培养基。
In order to enhance germination rate of Murraya paniculata seeds, the best treating time of concentrated sulfuric acid for seeds and effects of different hormone treatments on natural germination of M. paniculata seeds were researched, and the effects of different mass concentrations of 6-BA, NAA, IBA, and GA3 on explants growing and the effects of different mass concentrations of activated carbon on explants browning were researched, using intact seed and cotyledon with embryo as explants. The results showed that the best treatment time of concentrated sulfuric acid was 1 min. The seeds treated with 200× 10^-6 IBA had the highest germination rate, up to 50%. Followed by the seeds treated with 50 × 10^- 6 and 150 × 10^- 6 NAA, and germination rates of both treatments were 45%. The explants in the media added with 2.5 mg/L 6-BA, 0.2 mg/L IBA, 0.1 mg/L GA3, or 0.2 mg/L NAA had higher induction rates, and the explants in the medium added with 1.5 g/L activated carbon had the lowest browning degree. The best medium for tissue culture ofM. paniculata was MS added with 2.5 mg/L 6-BA, 0.1 mg/L GA3, 0.2 mg/L NAA, and 1.5 g/L activated carbon.