文献类型：期刊文献

英文题名：Reference gene selection for quantitative real-time polymerase chain reaction in Populus

作者：Xu, Meng[1,2] Zhang, Bo[1,2] Su, Xiaohua[3] Zhang, Shougong[3] Huang, Minren[1,2]

第一作者：Xu, Meng

机构：[1] Jiangsu Key Lab. for Poplar Germplasm Enhancement and Variety Improvement, Nanjing Forestry University, Nanjing 210037, China; [2] Key Laboratory of Genetics and Biotechnology, Ministry of Education, Nanjing Forestry University, Nanjing 210037, China; [3] Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China

年份：2011

卷号：408

期号：2

起止页码：337-339

外文期刊名：Analytical Biochemistry

收录：EI(收录号：20201908623289);Scopus(收录号：2-s2.0-78149416568)

语种：英文

外文关键词：Polymerase chain reaction - Chains - Proteins

摘要：Accurate quantification of gene expression with quantitative real-time polymerase chain reaction (qRT-PCR) relies on the choice of an appropriate reference gene. In this study, nine candidate reference genes were selected to study the expression stability for qRT-PCR normalization in adventitious rooting of Populus hardwood cuttings. geNorm, NormFinder, and BestKeeper analysis revealed that actin isoform B (ACT) was the most unstable gene across developmental stages, whereas elongation factor 1 alpha (EF1a) and 18S recombinant RNA (18S) emerged as the most appropriate reference genes for qRT-PCR analysis in this complex developmental process. ? 2010.