文献类型：期刊文献

英文题名：A novel transcription factor SgTCP24 positively regulates mogroside V biosynthesis by activating SgSQE, SgCS, and SgCYP87D18 expression in Siraitia grosvenorii

作者：Mu, Detian[1,2] Shao, Yingying[1,2] Zhao, Huan[3] Luo, Zuliang[2,4] Mo, Changming[2,5] Wilson, Iain W.[6] Pan, Limei[7] Huang, Huaxue[8] Chen, Wenqiang[1] Lin, Xiaodong[1] Zhou, Huimin[1] Lu, Ying[1,2] Qiu, Deyou[9] Ma, Xiaojun[3] Tang, Qi[1,2]

第一作者：Mu, Detian

通信作者：Tang, Q[1];Mo, CM[2];Tang, Q[2];Ma, XJ[3]

机构：[1]Hunan Agr Univ, Coll Hort, Natl Res Ctr Engn Technol Utilizat Bot Funct Ingre, Changsha 410128, Peoples R China;[2]Yuelushan Lab, Changsha 410128, Peoples R China;[3]Capital Med Univ, Sch Tradit Chinese Med, Beijing 100069, Peoples R China;[4]Chinese Acad Med Sci & Peking Union Med Coll, Inst Med Plant Dev, State Key Lab Qual Ensurance & Sustainable Use Dao, Beijing 100193, Peoples R China;[5]Guangxi Acad Agr Sci, Guangxi Crop Genet Improvement & Biotechnol Lab, Nanning 530007, Peoples R China;[6]CSIRO Agr & Food, Canberra, ACT 2601, Australia;[7]Guangxi Key Lab High Qual Format & Utilizat Dao Di, Guangxi Bot Garden Med Plants, Nanning 530023, Peoples R China;[8]Hunan Huacheng Biotech Inc, Changsha 410205, Peoples R China;[9]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China

年份：2025

卷号：234

外文期刊名：INDUSTRIAL CROPS AND PRODUCTS

收录：;EI(收录号：20252818776249);Scopus(收录号：2-s2.0-105010326815);WOS:【SCI-EXPANDED(收录号:WOS:001532741900003)】；

基金：This work was supported by the National Key R&D Program Projects [grant number 2023YFD1200200]; the National Natural Science Foundation of China [grant numbers 32270398, U20A2004, 82173915, 82104548]; the Interdisciplinary Research Youth Guidance Project of Hunan Agricultural University [grant number 2024XKJC02]; the Key Project of Hunan Provincial Science & Technology Department [grant number 2022NK2004]; the Key Scientific Research Fund of Hunan Provincial Education Department [grant number 24A0168]; and the Postgraduate Scientific Research Innovation Project of Hunan Province [grant number CX20240062].

语种：英文

外文关键词：Siraitia grosvenorii; genome-wide; mogroside V; SgTCP24; key enzyme genes

摘要：Siraitia grosvenorii, a important economic crop is now used as a sweetener because its bioactive compound, mogroside V, has high sweetness with zero calories. However, mogroside V only accumulates in S. grosvenorii fruits, and its content is low. Enhancing the production of mogroside V has become a major commercial and scientific endeavor. TEOSINTE BRANCHED 1/CYCLOIDEA/PROLIFERATING CELL FACTOR (TCP) transcription factors (TFs) have a key role in regulating secondary metabolite biosynthesis, however, their role in regulating mogroside V is unclear. In this study, twenty-four SgTCP genes were identified based on genome-wide analysis and classified into two subgroups according to phylogenetic analysis. Based on the expression levels in different fruit development stages, co-expression network showed that SgTCP24 had a strong positive correlation with SgSQE, SgCS, and SgCYP87D18. Through the subcellular localization, yeast one hybrid, dual-luciferase, and electrophoretic mobility shift assay it was shown that SgTCP24 can directly bind and activate the expression of these three genes. When SgTCP24 was over-expressed in S. grosvenorii fruits, the production of mogroside V increased. Conversely, the content of mogroside V decreased in lines subjected to virus induced silencing. Our results provide a major insight into the molecular mechanism of TCP TFs in regulating the mogroside V biosynthesis, and offers a new method for enhancing the content of mogroside V in plants.