文献类型：期刊文献

中文题名：由臭镰孢Fusarium foetens侵染引起的油桐根腐病

英文题名：Fusarium foetens,the pathogen of Fusarium root rot of Vernicia fordii

作者：王嘉[1,2] 杨安仁[3] 刘北平[1] 高暝[1] 赵耘霄[1] 陈益存[1] 汪阳东[1]

第一作者：王嘉

机构：[1]中国林业科学研究院亚热带林业研究所全省林木育种重点实验室,浙江杭州311400;[2]南京林业大学,江苏南京210037;[3]贵州鸿发生态农业科技有限责任公司,贵州黔南558200

年份：2026

卷号：45

期号：2

起止页码：73-84

中文期刊名：菌物学报

外文期刊名：Mycosystema

收录：;北大核心:【北大核心2023】；

基金：国家自然科学基金(32572095);浙江省万人计划科技创新领军人才项目(2022R52028)。

语种：中文

中文关键词：油桐;根腐病;臭镰孢;形态特征;分子鉴定

外文关键词：Vernicia fordii;Fusarium root rot;Fusarium foetens;morphological characteristics;molecular identification

分类号：S763.7

摘要：在贵州独山发现一种油桐根腐病,以生长旺盛期5月至7月发生最严重,发病部位在油桐根部,罹病植株叶片萎蔫、失水褪绿,最后干枯死亡。采用微生物群落真菌ITS高通量测序对健康油桐和发病油桐根际土壤的真菌群落组成进行了分析,它们的主要优势菌门和菌属组成相近,但相对丰度却大不相同,并且能广泛引起植物病害的镰孢属Fusarium只在发病油桐根际土壤菌群中出现,相对丰度占比为2.44%;采用组织分离法对引起油桐根腐病的病原菌进行分离纯化,从发病油桐根茎的病健交界处获得10株分离菌株,其中菌株AX-2回接到油桐幼苗后,呈典型的根腐病发病症状,进一步从发病植株的根茎部重新分离获得病原菌,与田间采集分离的病原菌形态特征一致,通过分子鉴定确定了该菌株与AX-2是同一种菌株。经形态学观察和多基因联合建树分析(ITS+tef1-a+rpb2+CaM+tub2),菌株AX-2被鉴定为臭镰孢Fusariumfoetens。这是该菌所致油桐根腐病的首次发现,为油桐根腐病的侵染特性及防控技术研究提供了理论依据。
A new disease was found on Vernicia fordii with symptom of root rot in Dushan,Guizhou Province.The disease occurred most seriously at growth stage(May to July),primarily affecting the roots.The diseased plants initially showed wilting and leaves fade green to withered until plant death.The fungal community composition of the rhizosphere soil was analyzed from healthy and diseased plants.The result showed that mainly dominant phyla and genera in the rhizosphere soil of diseased and healthy plants were similar,but the relative abundance was absolutely different.Moreover,the genus Fusarium causing a wide range of plant diseases only appeared in the fungal community of rhizosphere soil of diseased V.fordii,with relative abundance accounting for 2.44%.The pathogens were isolated and purified through tissue isolation.Ten strains were isolated from the healthy junction of V.fordii rhizome suffering from typical root rot.Among them,strain AX-2 induced typical symptoms of root rot after being back-inoculated to the seedlings of V.fordii.The pathogen was subsequently isolated from the roots and stems of diseased plants.Its morphological characteristics were found to be consistent with those of the pathogen isolated from the field.Molecular identification further verified that this strain was identical to AX-2.Morphological characteristics and multi-loci(ITS+tef1-a+rpb2+CaM+tub2)phylogeny classified AX-2 as Fusarium foetens.The species is firstly reported as the pathogen of root rot in V.fordii.This study provides a theoretical foundation for investigating the infection characteristics and control strategies of root rot disease in V.fordii.