文献类型：期刊文献

英文题名：Increasing the Carbohydrate Output of Bamboo Using a Combinatorial Pretreatment

作者：Huang, Chen[1,2,3] Fang, Guigan[1,2] Zhou, Yang[2] Du, Xinghu[2] Yu, Longxiang[2] Meng, Xianzhi[3] Li, Mi[4] Yoo, Chang Geun[5,6] Chen, Bingwei[7] Zhai, Shengcheng[7] Guan, Qiyuan[1] Yong, Qiang[2] Ragauskas, Arthur J.[3,4,5]

第一作者：黄晨;Huang, Chen

通信作者：Fang, GG[1];Fang, GG[2];Ragauskas, AJ[3];Ragauskas, AJ[4];Ragauskas, AJ[5]|[a000589b6171897e30b38]房桂干;

机构：[1]Chinese Acad Forestry, Inst Chem Ind Forest Prod, Jiangsu Prov Key Lab Biomass Energy & Mat, Nanjing 210042, Peoples R China;[2]Nanjing Forestry Univ, Coinnovat Ctr Efficient Proc & Utilizat Forest Re, Nanjing 210037, Peoples R China;[3]Univ Tennessee, Dept Chem & Biomol Engn, Knoxville, TN 37996 USA;[4]Univ Tennessee, Dept Forestry Wildlife & Fisheries, Ctr Renewable Carbon, Inst Agr, Knoxville, TN 37996 USA;[5]Oak Ridge Natl Lab, UTK ORNL Joint Inst Biol Sci, Biosci Div, Oak Ridge, TN 37831 USA;[6]SUNY Coll Environm Sci & Forestry, Dept Paper & Bioproc Engn, Syracuse, NY 13210 USA;[7]Nanjing Forestry Univ, Coll Mat Sci & Engn, Nanjing 210037, Peoples R China

年份：2020

卷号：8

期号：19

起止页码：7380-7393

外文期刊名：ACS SUSTAINABLE CHEMISTRY & ENGINEERING

收录：;EI(收录号：20202008661801);Scopus(收录号：2-s2.0-85084571791);WOS:【SCI-EXPANDED(收录号:WOS:000535305600015)】；

基金：The authors acknowledge the Jiangsu Province Key Laboratory of Biomass Energy and Materials (No. JSBEM-S-202004), Innovative Ability Enhancement Project for R&D Team of ICIFP, CAF (LHSXKQ8), and the Fundamental Research Funds of CAF "Enhancing Project in the Field of Wood Composite Materials and Chemical Resources Utilization" (CAFYBB2017ZX003-08) for supporting the work presented in this paper.

语种：英文

外文关键词：bamboo; combinatorial pretreatments; enzymatic hydrolysis; xylo-oligosaccharides; lignin

摘要：Bamboo represents a family of highly lignified biomass with significant resistance to cellulolytic enzyme degradation, and typically, a severe thermochemical pretreatment is needed to overcome its recalcitrance for producing fermentable sugars. Combinatorial pretreatments with a low holding temperature and low alkali dosage were investigated in this study in an attempt to remove the impediments to the accessibility of bamboo cellulose to enzymes and enable the efficient conversion of hemicellulose. Upon autohydrolysis at 180 degrees C, more than half of the hemicellulose (similar to 58%) was solubilized, contributing to further delignification in the subsequent dilute alkali extraction (DAE). The solubilized hemicellulose mainly existed in the form of soluble xylan, which, after a novel endo-beta-1,4-xylanase hydrolysis, generated a large amount of xylo-oligosaccharides with the xylobiose and xylotriose proportion reaching 72.99%. After the fractionation with combinatorial pretreatments, cellulose and xylose enzymatic hydrolysis yields were 73.36 and 86.98%, respectively, representing increments of 22.42 and 43.15% compared with the sole autohydrolysis process. Fluorescence microscopy (FM) and confocal Raman microscopy (CRM) images revealed that these dramatic improvements were caused by the deconstruction of the bamboo cell walls, including separation of the adjacent cell walls, removal of hemicellulose and lignin, migration and redistribution of lignin, and the increased exposure of cellulose. In addition, lignin chemical structure variations during the combinatorial pretreatment were analyzed with gel permeation chromatography (GPC), 2D HSQC NMR, and P-31 NMR, and the correlation between the lignin structure and enzymatic hydrolysis efficiency was investigated. In summary, this study highlighted the application of combinatorial pretreatments to maximize the carbohydrate output of bamboo and unveiled their mechanism that enabled the high-efficiency utilization of bamboo.