文献类型：期刊文献

英文题名：The Analysis Of Transcriptome and Metabolome Suggested an Association between the Self-Incompatibility of Acacia Mangium And 2 Si-Related Genes and 3 Metabolites

作者：Zhang, Ruping[1] Huang, Liejian[2] Zeng, Bingshan[1]

第一作者：Zhang, Ruping

机构：[1] Chinese Academy of Sciences, China; [2] State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of State Forestry Administration on Tropical Forestry, Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou, 510520, China

年份：2022

外文期刊名：SSRN

收录：EI(收录号：20220444484)

语种：英文

外文关键词：Amino acids - Biomolecules - Cultivation - Fluorescence - Fluorescence microscopy - Metabolism - Metabolites - Proteins

摘要：The type and molecular mechanism of self-incompatibility (SI) in Acacia mangium are unknown, seriously inhibiting the progress of breeding programs for this species. To confirm the SI type of A. mangium, pollen germination was observed by fluorescence microscopy at 0 h, 3 h, 6 h, 9 h, 12 h, and 24 h after self-pollination. To further explore the SI molecular mechanism of A. mangium, transcriptome and metabolome analyses were carried out at 0 h and 24 h after self-pollination. The observation of pollen germination after self-pollination using fluorescence microscopy speculated that the SI type of A. mangium is gametophytic self-incompatibility (GSI). The combined transcriptome and metabolome analysis yielded genes related to SI (all were enriched differentially expressed genes): 6 S-glycoproteins, 93 F-box proteins, and 69 26 S proteasomes. There were 38 calcium-dependent protein kinases/calmodulins and 41 thioredoxins. These genes were significantly enriched in KEGG pathways sulfur metabolism, tyrosine metabolism, phenylalanine metabolism, butyrate metabolism, and valine, leucine and isoleucine degradation. Through the analysis of these six leucine and isoleucine degradation pathways, it was found that the differentially expressed SI genes were enriched, corresponding to succinic acid, methylmalonic acid and 3-hydroxypropane. These three esters significantly downregulated the metabolites, and succinic acid was the metabolite. We speculate that this 2 genes and 3 metabolites are an important factor that causes pollen tube to fail to germinate and eventually leads to SI occurrence in A. mangium. ? 2022, The Authors. All rights reserved.