文献类型：期刊文献

英文题名：METHODS TO PROMOTE FRUIT FORMATION AND ASYMBIOTIC SEED GERMINATION OF CALANTHE TSOONGIANA T. TANG ET F. T. WANG

作者：Wang, Cai Xia[1] Tian, Min[1] Jiang, Ya Ting[1] Zhang, Ying[1]

第一作者：王彩霞

通信作者：Tian, M[1]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou 311400, Zhejiang, Peoples R China

年份：2018

卷号：18

期号：2

起止页码：49-57

外文期刊名：PROPAGATION OF ORNAMENTAL PLANTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000441226600002)】；

基金：This work was supported by the Key Science and Technology Specific Projects of Zhejiang Province (No. 2016C02056-13-2). We thank Xiaoling Niu (Administration Bureau of Tianmu Mount National Nature Reserve of Zhejiang Province) for assistance in artificial pollination in wild for this work.

语种：英文

外文关键词：artificial pollination; asymbiotic germination; NaOCl pretreatment; rare orchid; Calanthe tsoongiana

摘要：Calanthe tsoongiana T. Tang et F. T. Wang, a terrestrial orchid that is endemic to China, has become rare and endangered due to limited natural reproduction and habitat destruction. There is an urgent need to conserve this species, while it has been poorly studied. Methods to promote fruit formation and asymbiotic seed germination of C. tsoongiana were used. The fruit set of C. tsoongiana was only 2.5% in the natural habitats. Artificial pollination resulted in 100% of capsule formation and all capsules survived to full maturity by bagging treatment. The seeds collected at 150 days after pollination were the optimum for asymbiotic germination. Among the six researched basal media the earliest initiation of germination and the highest germination were observed on VW (50.5 days, 32.3%) and half-strength MS (50.2 days, 32.9%). No significant effect existed among three temperatures (15 +/- 2 degrees C, 20 +/- 2 degrees C, and 25 +/- 2 degrees C), while a 16 h photoperiod of 20 mu mol m(-2) s(-1) was better than a 16 h photoperiod of 56 mu mol m(-2) s(-1) or continuous darkness for seed germination and seedling formation of C. tsoongiana. In conclusion, the best germination (32.3-32.9%) was obtained in 150 DAP seeds that cultured on VW and half-strength MS medium under a 16 h photoperiod of 20 mu mol m(-2) s(-1) at 15 +/- 2 degrees C to 25 +/- 2 degrees C. Pretreatment with 1% sodium hypochlorite (NaOCl) for 40-60 min was effective in advancing the initial of germination and improving the germination.