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额尔齐斯河流域银白杨克隆结构及多样性研究     被引量:5

Clonal Structure and Clonal Diversity of Populus alba along the Erqis River

文献类型:期刊文献

中文题名:额尔齐斯河流域银白杨克隆结构及多样性研究

英文题名:Clonal Structure and Clonal Diversity of Populus alba along the Erqis River

作者:郑书星[1,2] 张建国[1] 段爱国[1] 何彩云[1] 保尔江[3] 王健[3]

第一作者:郑书星

机构:[1]中国林业科学研究院林业研究所,林木遗传育种国家重点实验室,国家林业局林木培育重点实验室;[2]中国林业科学研究院资源昆虫研究所;[3]阿勒泰地区林业科学研究所

年份:2013

卷号:26

期号:4

起止页码:426-432

中文期刊名:林业科学研究

外文期刊名:Forest Research

收录:CSTPCD;;Scopus;北大核心:【北大核心2011】;CSCD:【CSCD2013_2014】;

基金:中央级公益性科研院所基本科研业务费专项资金项目"额尔齐斯河天然杨树种群遗传多样性研究"(201004035)

语种:中文

中文关键词:银白杨;克隆结构;克隆多样性;SSR

外文关键词:Populus alba; clonal structure; clonal diversity; SSR

分类号:S718

摘要:新疆额尔齐斯河流域河谷的银白杨天然林分呈雌雄独立斑块状分布,为了解其成因及分布模式,利用SSR分子标记技术对根据2种采样策略采集的银白杨样本进行了克隆结构和克隆多样性研究。结果表明:(1)运用12对SSR引物对采自各雌雄独立斑块的所有样本进行分析,发现所有样本单株是同1个无性系克隆,斑块内只有1个基株,即斑块居群是单克隆结构;(2)整个流域不同斑块所采集的90个样本用12对SSR引物进行扩增分析,共检测到53个基因型(克隆基株),银白杨居群克隆多样性水平较高,Simpson指数(D)平均为0.983,基因型比率PD平均为0.589。银白杨以根孽方式繁殖后代,其最初建群时由多个不同基因型克隆基株形成了较高的克隆多样性,现存植株是克隆后代或克隆后代的后代。
The populations of Populus alba present a independently distributing pattern of male or female patch.To realize the form reason and distributing mode,we selected 12 microsatellite primers to study the clonal structure and clonal diversity of Populus alba base on two sampling strategy.The result showed that:(1) all swatches in a single distributing patch of male or female were a identical clone,the single patch had only one genet;(2) we differentiated 53 genotypes(genet) among the 90 plants sampled of all patches.The populations of Populus alba had an abundant clonal diversity and the mean Simpson’s index was 0.983,and mean PD was 0.589.The progeny of Populus alba was reproduced by root clone.The high clonal diversity might be maintained if the populations were initially founded by multiple genets that differed genetically and the existent frond was the progeny of clonal genets.

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