文献类型：期刊文献

英文题名：Small GTP-binding protein PdRanBP regulates vascular tissue development in poplar

作者：Li, Shaofeng[2] Huang, Qinjun[1] Zhang, Bingyu[1] Zhang, Jianhui[3,4] Liu, Xue[2] Lu, Mengzhu[1] Hu, Zanmin[5] Ding, Changjun[1] Su, Xiaohua[1]

通信作者：Su, XH[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Forestry Adm, Key Lab Tree Breeding & Cultivat,State Key Lab Tr, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Expt Ctr Forestry North China, Beijing 100023, Peoples R China;[3]N Carolina State Univ, Plants Human Hlth Inst, Dept Hort Sci, 600 Laureate Way, Kannapolis, NC 28081 USA;[4]Biomarker Technol Corp, Beijing 101300, Peoples R China;[5]Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China

年份：2016

卷号：17

期号：1

外文期刊名：BMC GENETICS

收录：;Scopus(收录号：2-s2.0-84978249344);WOS:【SCI-EXPANDED(收录号:WOS:000378844000002)】；

基金：This work was supported by grants from the National Natural Science Foundation of China (31400570) and Fundamental Research Funds for the Central Non-profit Research Institution of the Chinese Academy of Forestry (CAFYBB2014QB051).

语种：英文

外文关键词：Molecular cloning; Functional analysis; PdRanBP; Populus deltoides; Vascular tissue

摘要：Background: Previous research has demonstrated that ectopic expression of Ran-binding protein (RanBP) in Arabidopsis results in more axillary buds and reduced apical dominance compared to WT plants. However, the function of RanBP in poplar, which has very typical secondary growth, remains unclear. Here, the Populus deltoides (Marsh.) RanBP gene (PdRanBP) was isolated and functionally characterized by ectopic expression in a hybrid poplar (P. davidiana Dode x P. bolleana Lauche). Results: PdRanBP was predominantly expressed in leaf buds and tissues undergoing secondary wall expansion, including immature xylem and immature phloem in the stem. Overexpression of PdRanBP in poplar increased the number of sylleptic branches and the proportion of cells in the G2 phase of the cell cycle, retarded plant growth, consistently decreased the size of the secondary xylem and secondary phloem zones, and reduced the expression levels of cell wall biosynthesis genes. The downregulation of PdRanBP facilitated secondary wall expansion and increased stem height, the sizes of the xylem and phloem zones, and the expression levels of cell wall biosynthesis genes. Conclusions: These results suggest that PdRanBP influences the apical and radial growth of poplar trees and that PdRanBP may regulate cell division during cell cycle progression. Taken together, our results demonstrated that PdRanBP is a nuclear, vascular tissue development-associated protein in P. deltoides.