文献类型：期刊文献

英文题名：Genome-wide identification of plasma membrane aquaporin gene family in Populus and functional identification of PIP1;1 involved in osmotic stress

作者：Jiang, Cheng[1,2] Song, Xueqin[2] He, Hui[2] Chu, Liwei[2] Zhou, Houjun[2] Zhao, Yanqiu[2] Xu, Yajing[1] Zeng, Wei[1] Lin, Xinchun[1] Lu, Meng-Zhu[1,2]

第一作者：Jiang, Cheng

通信作者：Lu, MZ[1]

机构：[1]Zhejiang A&F Univ, Sch Forestry & Biotechnol, State Key Lab Subtrop Silviculture, Hangzhou 311300, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China

年份：2020

卷号：179

外文期刊名：ENVIRONMENTAL AND EXPERIMENTAL BOTANY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000571483200002)】；

基金：This research was supported by the Basic Research Fund of Research Institude of Forestry, Chinese Academy of Forestry (RIF-2014-08).

语种：英文

外文关键词：Populus; Plasma membrane aquaporin; Regulation; Gene expression; Osmotic stress

摘要：Aquaporins are the membrane channels that facilitate the transportation of water and small neutral molecules in plants. As such, they play an important role in stress tolerance. This function may be more pronounced in woody plants, which must efficiently absorb water and transport it over long distances. Therefore, a functional analysis of plasma membrane aquaporins (PIP) under drought stress can provide insights into the roles of these proteins in woody plants. In this work, we obtained 15 members of the PtPIP gene family in poplar. They formed two groups in a phylogenetic analysis. Gene structure, including intron phases, was found to be remarkably well conserved among members of this gene family. Five paralogous gene pairs were identified in the PtPIP family, and four of those pairs arose via whole genome duplication. A selection pressure analysis showed that PtPIP genes have undergone positive selection. The transcripts of six PtPIP genes including PtPIP1;1 were predominantly detected in the root. Transgenic poplar overexpressing PtoPIP1;1 was sensitive to osmotic stress, which phenocopied PagMYB216-overexpressing poplar plants. Transactivation and yeast one-hybrid assays showed that PagMYB216, whose homologs in other plants are induced by abiotic stresses, is able to activate PtoPIP1;1 expression through binding to the MBS motif in its promoter. Our results provide new information about PtoPIPs and their roles in response to abiotic stresses, which will be helpful for better understanding their functions in Populus.