文献类型：期刊文献

英文题名：Microbacterium nymphoidis sp. nov. and Microbacterium festucae sp. nov., two novel species with high plant-promoting potential isolated from wetland plants in China

作者：Li, Chunyi[1] Jin, Xiaoqian[2,3] Yang, Fu[2] Zhao, Jiayi[2] Wang, Siyu[2] Sun, Qiwu[2] Li, Lubin[2] Liu, Lei[2]

通信作者：Liu, L[1]

机构：[1]Chinese Acad Forestry, Inst Ecol Conservat & Restorat, Beijing, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, Key Lab Tree Breeding & Cultivat Natl Forestry &, Beijing, Peoples R China;[3]Rizhao Garden & Sanitat Grp Co Ltd, Rizhao, Shandong, Peoples R China

年份：2023

卷号：73

期号：11

外文期刊名：INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001140942400010)】；

基金：This research was funded by the Fundamental Research Funds for the Central Non-profit Research Institution of Chinese Academy of Forestry (CAFYBB2020SZ006).

语种：英文

外文关键词：Microbacterium nymphoidis; Microbacterium festucae; Microbacterium; polyphasic taxonomy; plant-growth promoting

摘要：Two novel plant growth-promoting, rod-shaped, Gram-positive and non-motile rhizobacteria, W1N(T) and W2R(T), were isolated from wetland plants Festuca elata and Nymphoides peltatum, respectively, in China. The results of the 16S rRNA sequence alignment analysis showed that they were related to Microbacterium, with the highest similarity to Microbacterium ketosireducens (98.7%) and Microbacterium laevaniformans (98.5%) for strain W1N(T), and to Microbacterium terricola (98.1%) and Microbacterium marinum (98.0%) for strain W2R(T). Phylogenetic analyses based on 16S rRNA gene sequences and 92 conserved concatenated proteins suggested that the two strains belong to the genus Microbacterium and were placed in two separate novel phylogenetic clades. The genome sizes of the two strains were 3.2 and 3.7 Mb, and the G+C contents were 71.7 and 68.5 mol%, respectively. The comparative genome results showed that the average nucleotide identity values between W1N(T) and W2R(T) and other species ranged from 73.5 to 83.6 %, and the digital DNA-DNA hybridization values ranged from 19.7 to 26.8 %. These two strains show physiological and biochemical features that differ from those of closely related species. Rhamnose, galactose and glucose were present in the characteristic sugar fractions of strains W1N(T) and W2R(T). The peptidoglycan of strains W1N(T) and W2R(T) contained the amino acids ornithine, alanine and aspartic acid. C-15:0 anteiso, C-17:0 anteiso and C-16:0 iso were the predominant cellular fatty acids in W1N(T) and W2R(T). Phosphatidylglycerol and diphosphatidylglycerol are major polar lipid components. Strain W1N(T) not only formed bacterial biofilms but also had the ability to solubilize phosphorus and produce indole-3-acetic acid. Strain W2R(T) had siderophore-producing and lignin-degrading properties. Based on their genetic and phenotypic characteristics, strains W1N(T) and W2R(T) were classified as novel bacteria in the genus Microbacterium and designated as Microbacterium festucae sp. nov. (type strain W1N(T)=ACCC 61807(T)=GDMCC 1.2966(T)=JCM 35339(T)) and Microbacterium nymphoidis sp. nov. (type strain W2R(T)=ACCC 61808(T)=GDMCC 1.2967(T)=JCM 35340(T)).