文献类型：期刊文献

中文题名：柑橘凤蝶滞育期间水溶性蛋白初步研究

英文题名：A Preliminary Study on Water-solubile Portein of Diapause-pupae of Papilio xuthus

作者：易传辉[1,2] 陈晓鸣[1] 史军义[1] 周成理[1]

第一作者：易传辉

机构：[1]中国林业科学研究院资源昆虫研究所,国家林业局资源昆虫培育与利用重点实验室,云南昆明650224;[2]云南林业职业技术学院,应用生态与职业教育研究所,云南昆明650224

年份：2010

卷号：25

期号：5

起止页码：102-107

中文期刊名：西北林学院学报

外文期刊名：Journal of Northwest Forestry University

收录：CSTPCD;;北大核心:【北大核心2008】；CSCD:【CSCD_E2011_2012】；

基金：国家林业局引进国际先进林业科学技术计划项目(2005-4-59);国家林业局引进国际先进林业科学技术计划项目(2008-4-68)

语种：中文

中文关键词：柑橘凤蝶;蛋白质;滞育;蛹;毛细管区带电泳

外文关键词：Papilio xuthus; diapause; protein; pupae; capillary zone electrophoresi

分类号：S763.42

摘要：为深入了解柑橘凤蝶滞育机理,利用毛细管区带电泳对滞育期间蛋白质特征进行了研究。电泳结果显示,滞育期间蛋白质吸收峰在18-45个之间,除10月下旬为18个最少和12月下旬为45最多外,其余时期相近,在22-24个之间,同时,不同时期蛋白质吸收峰在面积和迁移时间上存在差异。结果表明,滞育期间进行着一定程度的蛋白质代谢,种类、数量和含量发生了变化。12月下旬蛋白质种类和数量明显增加,含量也有所变化,与气温下降抗冻蛋白产生有关。滞育期间蛋白质特征与非滞育蛹和滞育蛹羽化成虫有明显差异,表明滞育期间有着与其他发育阶段不同的生命特征。
The water-solubile protein of the butterfly,Papilio xuthus was analyzed by capillary zone electrophoresis（CZE）.The results as follow： there were 18~45 electropherograms absorb peaks during the stage of diapause,in which the minimum peak number occurred in the last ten days of October（18）,and the maximum one was in the last ten days of December（45）,there were little difference in other stages： within 22 to 24.And at the same time,the areas and migration time of absorb peaks were different,indicating that the protein had some degree metabolism during the stage of diapause,the kinds,numbers and content of protein changed in some degrees.The kinds and numbers of protein rised,and the content changed due to some antifrozen proteins were formed to adapt low temperature during the last ten days of December.Comparing with the stage of non-diapause pupae and the adult eclosion from diapause pupae,the characteristics of protein during the diapause pupae were different obviously,indicating different characteristics of life at the stage of diapause from other stages.