文献类型：期刊文献

英文题名：Transcriptomics and Proteomics Reveal the Cellulose and Pectin Metabolic Processes in the Tension Wood (Non-G-Layer) of Catalpa bungei

作者：Xiao, Yao[1] Yi, Fei[1] Ling, Juanjuan[1] Wang, Zhi[1] Zhao, Kun[2] Lu, Nan[1] Qu, Guanzheng[3] Kong, Lisheng[4] Ma, Wenjun[1] Wang, Junhui[1]

第一作者：Xiao, Yao

通信作者：Wang, JH[1]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat, State Forestry Adm,Res Inst Forestry, Beijing 100091, Peoples R China;[2]Luoyang Acad Agr & Forestry Sci, Luoyang 471002, Peoples R China;[3]Northeast Forestry Univ, State Key Lab Tree Genet & Breeding, Harbin 150040, Peoples R China;[4]Univ Victoria, Ctr Forest Biol, Dept Biol, 3800 Finnerty Rd, Victoria, BC V8P 5C2, Canada

年份：2020

卷号：21

期号：5

外文期刊名：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000524908500137)】；

基金：This work was supported by the Fundamental Research Funds of Chinese Academy of Forestry (CAFYBB2017ZY002)

语种：英文

外文关键词：tension wood; transcriptome; proteomics; Raman spectroscopy; cellulose; pectin; Catalpa bungei

摘要：Catalpa bungei is an economically important tree with high-quality wood and highly valuable to the study of wood formation. In this work, the xylem microstructure of C. bungei tension wood (TW) was observed, and we performed transcriptomics, proteomics and Raman spectroscopy of TW, opposite wood (OW) and normal wood (NW). The results showed that there was no obvious gelatinous layer (G-layer) in the TW of C. bungei and that the secondary wall deposition in the TW was reduced compared with that in the OW and NW. We found that most of the differentially expressed mRNAs and proteins were involved in carbohydrate polysaccharide synthesis. Raman spectroscopy results indicated that the cellulose and pectin content and pectin methylation in the TW were lower than those in the OW and NW, and many genes and proteins involved in the metabolic pathways of cellulose and pectin, such as galacturonosyltransferase (GAUT), polygalacturonase (PG), endoglucanase (CLE) and beta-glucosidase (BGLU) genes, were significantly upregulated in TW. In addition, we found that the MYB2 transcription factor may regulate the pectin degradation genes PG1 and PG3, and ARF, ERF, SBP and MYB1 may be the key transcription factors regulating the synthesis and decomposition of cellulose. In contrast to previous studies on TW with a G-layer, our results revealed a change in metabolism in TW without a G-layer, and we inferred that the change in the pectin type, esterification and cellulose characteristics in the TW of C. bungei may contribute to high tensile stress. These results will enrich the understanding of the mechanism of TW formation.