文献类型：期刊文献

英文题名：Determination of reactive oxygen species generated in laccase catalyzed oxidation of wood fibers from Chinese fir (Cunninghamia lanceolata) by electron spin resonance spectrometry

作者：Zhou, Guanwu[1,2] Li, Jianing[1,2,3] Chen, Yongsheng[2] Zhao, Baolu[2] Cao, Yongjian[1] Duan, Xinfang[1] Cao, Yuanlin[2]

第一作者：Zhou, Guanwu

通信作者：Duan, XF[1]|[a0005dc0691661c4fbbc9]段新芳;

机构：[1]Chinese Acad Forestry, Res Inst Wood Ind, Beijing 100091, Peoples R China;[2]Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China;[3]NW A&F Univ, Coll Mech & Elect Engn, Yangling 712100, Shaanxi, Peoples R China

年份：2009

卷号：100

期号：1

起止页码：505-508

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000260152300079)】；

基金：This study was supported by the Project 30471352 from National Natural Science Foundation of China.

语种：英文

外文关键词：Reactive oxygen species (ROS); Electron spin resonance (ESR); Wood fibers; Laccase; Lignin

摘要：The aim of the present study was to determine whether the radical reaction intermediates - reactive oxygen species (RCS) were formed during the laccase-catalyzed oxidation of wood fibers from Chinese fir (Cunninghamia lanceolata) and to quantify tentatively its production with electron spin resonance (ESR) spectrometry. To investigate the activation pathways triggered by laccase, ESR spin-trapping techniques using N-tert-butyl-alpha-phenylnitrone (PBN) as spin trap followed by ethyl acetate extraction were employed to identify and quantify the free radical intermediates. ROS such as the superoxide and hydroxyl radical was detected and quantified in the laccase catalyzed oxidation of wood fibers, suggesting that ROS is the main free radical intermediates for laccase reaction. Based on the findings of the presence of ROS and previous literature on the free radical reaction of laccase oxidation of wood fibers, a possible reaction mechanism involving ROS-mediated attack on the domains of lignin which is not directly accessible for the enzyme and solubilized low-molecular mass lignins which function as reactive compounds like adhesives and may cling back to the fiber surface, could accordingly describe laccase-catalyzed oxidation of Chinese fir wood fibers. (c) 2008 Elsevier Ltd. All rights reserved.