文献类型：期刊文献

中文题名：镉胁迫诱导的杞柳组织离子组变异及其对镉运输的影响

英文题名：Cadmium Stress-Induced Variation in Ionome in Different Tissues of Salix integra and Its Impact on Cadmium Transport

作者：舒婉钦[1,3] 陈光才[1] 曹基武[3] 王树凤[1,2]

第一作者：舒婉钦

机构：[1]中国林业科学研究院亚热带林业研究所,杭州311400;[2]中国林业科学研究院林木遗传育种国家重点实验室,北京100091;[3]中南林业科技大学林学院,长沙410004

年份：2023

卷号：59

期号：6

起止页码：36-47

中文期刊名：林业科学

外文期刊名：Scientia Silvae Sinicae

收录：CSTPCD;;EI(收录号：20233814754105);Scopus;北大核心:【北大核心2020】；CSCD:【CSCD2023_2024】；

基金：国家自然科学基金项目(31770653)。

语种：中文

中文关键词：镉胁迫;杞柳;离子组;矿质元素;镉转运

外文关键词：cadmium stress;Salix integra;ionome;mineral elements;cadmium transport

分类号：S718.43

摘要：【目的】探究杞柳4个栽培品种在重金属镉(Cd)胁迫下的离子组响应特征,为选育高Cd富集型杞柳、提高杞柳对重金属污染土壤的修复效率提供理论依据。【方法】采用水培试验,分析杞柳4个品种‘大红头’、‘大青皮’、‘黄皮柳’、‘庄坞柳’在0、10、50μmol·L-1的Cd(NO3)2处理下根、插条、新枝和叶组织Cd、大量元素(N、P、K、Ca、Mg)、微量元素(Fe、Zn、Cu、Mn、Mo、Se)含量变化,结合主成分分析、相关性分析以及偏最小二乘回归分析,明确Cd胁迫下杞柳不同组织离子组变异的元素贡献以及影响杞柳Cd积累和转运的主要元素。【结果】1)大量元素特别是N的变异系数明显小于其他元素,叶片Cd变异系数明显高于其他组织;Mn、Zn和Fe等二价阳离子在Cd胁迫下的变异系数均较高。2)杞柳离子组总变异的78.3%由组织类型的差异导致,其中Zn、Fe、Cu、Mn和Mo是杞柳离子组变异的重要组成部分。3)Cd胁迫对不同组织离子组干扰程度为:根>叶>新枝>插条。4)相关性分析发现,叶片Cd含量与Fe和Mg呈显著正相关;新枝和插条Cd含量与N和Se呈显著正相关;根部Cd含量与Ca、Cu和Se呈显著正相关。聚类分析和偏最小二乘回归确定N、P、Ca、K、Mn、Fe、Mg和Mo等元素在杞柳转运Cd的过程中起重要作用。【结论】Cd胁迫导致的杞柳离子组变异,主要来源于组织类型的差异;同一组织中,离子组的变异主要来源于Cd胁迫,不同组织中引起离子组变异的主要元素各不相同。Cd在杞柳体内的转运受离子组变异影响,其中的大量元素N、P、K、Ca、Mg,微量元素Mn、Fe和Mo在杞柳转运Cd的过程中起重要作用。
【Objective】This study aims to investigate the ionomic responses in four cultivars of Salix integra under cadmium(Cd)stress,so as to provide a theoretical basis for selecting high Cd-enriched S.integra,and improving the remediation efficiency for heavy metal contaminated soil.【Method】A hydroponic experiment was conducted to analyze the variation of Cd,macro elements(N,P,K,Ca,Mg),and trace elements(Fe,Zn,Cu,Mn,Mo,Se)contents in roots,cuttings,new branches and leaves of four cultivars of S.integra under 0,10,and 50μmol·L?1 of Cd(NO3)2 treatments.Combined with principal component analysis,correlation analysis and partial least squares regression analysis were performed to identify the elemental contribution of ionomic variation in different tissues under Cd stress and influence on accumulation of Cd and translocation of the major elements in S.integra.【Result】1)The coefficient of variation(CV)of macro elements,especially N,was significantly smaller than that of the other elements,and the CV of Cd in leaf tissue was significantly higher than that in the other tissues.The CVs of divalent cations such as Mn,Zn and Fe under Cd stress were all greater.2)PCA analysis revealed that 78.3%of the total variation in the ionome of S.integra was caused by the tissue types,among which Zn,Fe,Cu,Mn and Mo were important components of the variation in the ionome of S.integra.3)The degree of interference of Cd treatments on different tissue ionome was:root>leaf>new branch>cutting.Results also showed that Cd content in the leaves was significantly and positively correlated with Fe and Mg;Cd content in the new branches and cuttings was significantly and positively correlated with N and Se;Cd content in the roots was significantly and positively correlated with Ca,Cu and Se.Cluster analysis and partial least squares regression confirmed that N,P,Ca,K,Mn,Fe,Mg and Mo played an important role in Cd translocation in S.integra.【Conclusion】The ionomic variation in S.integra caused by Cd stress mainly originates from the differences among tissue types.In the same tissue,the ionomic variation mainly originates from Cd stress.Moreover,the main elements that cause ionomic variation vary in different tissues.The transport of Cd in S.integra is affected by ionomic variation,among which the macro elements of N,P,K,Ca,Mg,and the trace elements of Mn,Fe and Mo play an important role in the transport of Cd in S.integra.