文献类型：期刊文献

英文题名：Genetic Diversity of the Critically Endangered Thuja sutchuenensis Revealed by ISSR Markers and the Implications for Conservation

作者：Liu, Jianfeng[1] Shi, Shengqing[1] Chang, Ermei[1] Yang, Wenjuan[1] Jiang, Zeping[1]

第一作者：刘建锋

通信作者：Jiang, ZP[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China

年份：2013

卷号：14

期号：7

起止页码：14860-14871

外文期刊名：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000322171700112)】；

基金：The authors are grateful to Zhengyu Liu, Mingbo Ren, Shiqiang Zhang and Jianxiu Wang for their assistance in filed observation and sample collection. We also sincerely thank Fagen Li for his enthusiastic assistance in data analysis. Thanks also go to two anonymous reviewers for valuable suggestions and comments. This study was supported by the special fund of the Research Institute of Forestry, Chinese Academy of Forestry (grant RIF2010-15) and the 948 program of the State Forestry Administration of China (grant 2013-4-47)

语种：英文

外文关键词：Thuja sutchuenensis; genetic variation; ISSR markers; conservation implications

摘要：Thuja sutchuenensis Franch. is a critically endangered plant endemic to the North-East Chongqing, China. Genetic variation was studied to assess the distribution of genetic diversity within and among seven populations from the single remnant locations, using inter-simple sequence repeat (ISSR) markers. A total of 15 primers generated 310 well defined bands, with an average of 20.7 bands per primer. The seven populations revealed a relatively high level of genetic diversity in the species. The percentage of polymorphic bands, Nei's gene diversity and Shannon's information index at the population and species level were 76.1%, 0.155, 0.252 and 100%, 0.165, 0.295, respectively. A low level of genetic differentiation among populations (G(ST) = 0.102), in line with the results of Analyses of Molecular Variance (AMOVA), and a high level of gene flow (N-m = 4.407) were observed. Both the Unweighted Pair Group Method with Arithmatic Mean (UPGMA) cluster analysis and Principal Coordinates Analysis (PCoA) supported the grouping of all seven populations into two groups. In addition, Mantel test revealed no significant correlation between genetic and geographical distances (r = 0.329, p = 0.100). The low genetic differentiation among populations implies that the conservation efforts should aim to preserve all the extant populations of this endangered species.