文献类型：期刊文献

中文题名：杂交杏李‘风味玫瑰’花芽分化过程及内源激素动态变化

英文题名：Flower bud differentiation and dynamic changes of endogenous hormone in Prunus domestica×armeniaca ’Fengweimeigui’

作者：莫文娟[1,2] 张嘉嘉[3] 杨绍彬[3] 许言[1] 苑晨光[1] 刘亚心[4]

第一作者：莫文娟

机构：[1]中国林业科学研究院华北林业实验中心,北京102300;[2]康奈尔大学综合植物科学院,美国纽约14853;[3]中国林业科学研究院经济林研究开发中心/经济林种质创新与利用国家林业和草原局重点实验室,郑州450003;[4]河南农业大学林学院,郑州450002

年份：2020

期号：11

起止页码：54-61

中文期刊名：中国农业大学学报

外文期刊名：Journal of China Agricultural University

收录：CSTPCD;;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；

基金：中国林科院基本科研业务费专项资金项目(CAFYBB2017MB018)

语种：中文

中文关键词：杂交杏李;‘风味玫瑰’;花芽分化;内源激素;动态变化

外文关键词：Prunus domestica×armeniaca;’Fengweimeigui’;flower bud differentiation;endogenous hormones;dynamic changes

分类号：S662.3

摘要：为了解内源激素对杂交杏李花芽分化的影响,以‘风味玫瑰’为研究对象,采用石蜡切片法对其花芽进行解剖观察,用高效液相色谱—质谱联用(LC-MS/MS)法测定花芽分化过程中内源激素含量。结果表明:‘风味玫瑰’7月中下旬进入花原基分化期,8月下旬进入萼片原基分化期,9月下旬进入花瓣原基分化期,10月下旬进入雌、雄蕊原基分化期;花芽分化过程中生长素(IAA)含量呈现先下降后保持平稳的趋势,脱落酸(ABA)含量呈先上升后下降再急剧上升变化趋势,赤霉素(GA3)含量总体呈现"降-升-降"的变化,即先逐步下降后急剧上升再急剧下降,茉莉酸甲酯(MeJA)含量呈现逐渐下降的趋势。综上,低水平IAA、GA3、MeJA和高水平ABA有利于花芽分化,且较高的mABA/mGA3和mABA/mMeJA比值有利于花芽分化。
In order to study the effect of endogenous hormone on flower bud differentiation of Prunus domestica×armeniaca.‘Fengweimeigui’was selected as the experimental material.The paraffin cross section method was used to investigate flower bud morphological differentiation.High performance liquid chromatography-mass spectrometry(LC-MS/MS)was used to measure the endogenous hormone levels during flower bud differentiation.The results showed that:P.domestica×armeniaca‘Fengweimeigui’entered the stage of floral primordial differentiation in the middle of July,the Sepal primordial differentiation stage in late August,the petal primordial differentiation stage in late September,and Stamen&Pistil primordial differentiation stage in October.During flower bud differentiation,indole-3-aceticacid(IAA)content reached a stable status after the stage of floral primordial differentiation.Abscisic acid(ABA)content followed a pattern of increase-decrease-increase,whereas gibberellic acid(GA3)content was opposite to abscisic acid,showing a pattern of decrease-increase-decrease.Methyl jasmonate(MeJA)was significantly decreased during flower bud differentiation.In conclusion,the low levels of IAA,GA3,MeJA and high levels of ABA were favorable for flower bud differentiation.In addition,high mABA/mGA3 and mABA/mMeJAratios were favorable for flower bud differentiation.