文献类型：期刊文献

英文题名：Growth-regulating factor 5 (GRF5)-mediated gene regulatory network promotes leaf growth and expansion in poplar

作者：Wu, Wenqi[1] Li, Jiang[1] Wang, Qiao[2] Lv, Kaiwen[3] Du, Kang[1] Zhang, Wenli[4] Li, Quanzi[2] Kang, Xiangyang[1] Wei, Hairong[5]

第一作者：Wu, Wenqi

通信作者：Kang, XY[1];Wei, HR[2]

机构：[1]Beijing Forestry Univ, Beijing Adv Innovat Ctr Tree Breeding Mol Design, Beijing 100083, Peoples R China;[2]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[3]Northeast Forestry Univ, State Key Lab Tree Genet & Breeding, Harbin 150040, Heilongjiang, Peoples R China;[4]Nanjing Agr Univ, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Jiangsu, Peoples R China;[5]Michigan Technol Univ, Coll Forest Resources & Environm Sci, Houghton, MI 49931 USA

年份：2021

卷号：230

期号：2

起止页码：612-628

外文期刊名：NEW PHYTOLOGIST

收录：;Scopus(收录号：2-s2.0-85101022833);WOS:【SCI-EXPANDED(收录号:WOS:000617752300001)】；

基金：This study was supported by the National Natural Science Foundation of China (31530012) to XK. The startup funding for polyploid poplar research was from 'Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University'. The authors thank Professor Chenggui Han (China Agricultural University) and Professor Haiyang Wang (South China Agricultural University) for their kind help. The authors declare no conflict of interest. The authors thank Dongmei Hu for helping to prepare Fig. S1 and Dr Jennifer Sanders for proofreading the manuscript.

语种：英文

外文关键词：cytokinin; gene regulatory network; growth‐ regulating factor; leaf growth; leaf size; Populus; triploid

摘要：Although polyploid plants have larger leaves than their diploid counterparts, the molecular mechanisms underlying this difference (or trait) remain elusive. Differentially expressed genes (DEGs) between triploid and full-sib diploid poplar trees were identified from two transcriptomic data sets followed by a gene association study among DEGs to identify key leaf growth regulators. Yeast one-hybrid system, electrophoretic mobility shift assay, and dual-luciferase assay were employed to substantiate that PpnGRF5-1 directly regulated PpnCKX1. The interactions between PpnGRF5-1 and growth-regulating factor (GRF)-interacting factors (GIFs) were experimentally validated and a multilayered hierarchical regulatory network (ML-hGRN)-mediated by PpnGRF5-1 was constructed with top-down graphic Gaussian model (GGM) algorithm by combining RNA-sequencing data from its overexpression lines and DAP-sequencing data. PpnGRF5-1 is a negative regulator of PpnCKX1. Overexpression of PpnGRF5-1 in diploid transgenic lines resulted in larger leaves resembling those of triploids, and significantly increased zeatin and isopentenyladenine in the apical buds and third leaves. PpnGRF5-1 also interacted with GIFs to increase its regulatory diversity and capacity. An ML-hGRN-mediated by PpnGRF5-1 was obtained and could largely elucidate larger leaves. PpnGRF5-1 and the ML-hGRN-mediated by PpnGRF5-1 were underlying the leaf growth and development.