文献类型：期刊文献

英文题名：Characterization and structural analysis of the coat protein from Chinese isolates of Cymbidium mosaic virus

作者：Liu, Yun-Chao[1,3] Li, Lu-Bin[2] Li, Yun-Liang[1,3] Ma, Qing-Hu[1] Peng, Zhen-Hua[2]

第一作者：Liu, Yun-Chao

通信作者：Ma, QH[1]

机构：[1]Chinese Acad Sci, Inst Bot, Key Lab Photosynth & Environm Mol Physiol, Beijing, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, Lab Silviculture State Forestry Adm, Beijing, Peoples R China;[3]Chinese Acad Sci, Grad Sch, Beijing, Peoples R China

年份：2009

卷号：116

期号：6

起止页码：241-246

外文期刊名：JOURNAL OF PLANT DISEASES AND PROTECTION

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000273454900001)】；

基金：This work was supported by the National Natural Science Foundation of China (No. 30671043 and No. 30671134), the Natural Science Foundation of Beijing (No.6082018), the National Importation Program of China ("948" program, No. 2005-4-37), and the National High Technology and Research Development Program of P. R. China ("863" project, No. 2007AA10Z101).

语种：英文

外文关键词：orchids; phylogenetic reconstruction; plant virus; tetratricopeptide repeat

摘要：Cymbidium mosaic virus (CymMV) is the most prevalent virus to infect orchids. The coat protein of CymMV (CyCP) is not only necessary for encapsidating the virus RNA and cell-to-cell movement, but also a target to create virus-resistant transgenic orchids. in this report, a CymMV coat protein cDNA (designated as CyCP-CX) was isolated from Chinese isolates of Cymbidium mosaic virus. An extensive search in GenBank database and alignment analysis revealed another 49 CyCP isoforms from the orchids in six different countries. These genes plus CyCP-CX were used for phylogenetic reconstruction, which revealed three groups, namely group I, II and III. The CyCP sequences from the same country were generally not clustered together. Furthermore, the CyCP sequences from the same region or host plant also showed a high divergence. These data indicate that the CymMV has been frequently exchanged among the different countries as a result of the trade of orchids. Structural analysis indicates that the CyCP is similar to the tetratricopeptide repeat (TPR) protein with predicted three tandem TPR repeats. These TPR domains in CyCP structure may have significance in viral disassembly and propagation.