文献类型：期刊文献

中文题名：楸树COBRA家族分析及在应拉木形成中的转录响应

英文题名：Characterization of the COBRA Gene Family in Catalpa bungei and Its Transcriptional Response in Tension Wood Formation

作者：姚程程[1] 王瑞[1] 吴创业[2] 王青青[2] 胡继文[1] 刘冰洋[1] 费越[1] 卢楠[1] 王军辉[1]

第一作者：姚程程

机构：[1]林木遗传育种全国重点实验室国家林业和草原局林木培育重点实验室中国林业科学研究院林业研究所,北京100091;[2]温县林业科学研究所,河南焦作454850

年份：2025

卷号：38

期号：6

起止页码：48-58

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：;北大核心:【北大核心2023】；

基金：中央级公益性科研院所基本科研业务费(CAFYBB2021QB002)省市级。

语种：中文

中文关键词：楸树;应拉木;COBRA基因家族;基因表达;RNA测序

外文关键词：Catalpa bungei;tension wood;COBRA gene family;gene expression;RNA sequencing

分类号：S718.46

摘要：[目的]本研究旨在对楸树COBRA基因家族成员(CbuCOB)进行全基因组水平鉴定和系统表征,解析其在应拉木(TW)形成过程中的转录响应模式。[方法]运用生物信息学手段对CbuCOB基因进行鉴定和系统分析,利用RNA测序检测CbuCOB基因在不同组织中的表达特征及在楸树TW形成过程中的表达响应模式,并通过实时荧光定量(qRT-PCR)验证关键CbuCOB基因的相对表达量。[结果]楸树基因组中共鉴定到14个CbuCOB基因,这些基因在染色体分布不均。系统发育分析将CbuCOB蛋白分为2个亚家族(SFⅠ和SFⅡ)和12个亚组,同一亚组成员在结构上高度相似。启动子顺式作用元件分析显示,CbuCOB基因含有多个与生长发育、环境胁迫响应和植物激素信号传导相关的元件。组织表达分析表明CbuCOB基因在不同组织中表达具有特异性,其中CbuCOB1、CbuCOB2、CbuCOB3和CbuCOB9在木质部中表达水平较高。时序转录组分析显示CbuCOB基因在TW形成过程中表现出动态的转录响应,其中5个SFⅠ成员(CbuCOB1、CbuCOB2、CbuCOB8、CbuCOB9和CbuCOB12)和1个SFⅡ成员(CbuCOB4)被鉴定为差异表达基因。qRT-PCR验证了关键CbuCOB基因表达趋势与转录组结果相一致,这些基因可能参与调控楸树TW的形成。[结论]楸树COBRA基因家族成员的基因特性和表达模式呈现多样性,部分成员可能在楸树木材形成,尤其是TW形成过程中发挥关键调控作用。
[Objective]This study aimed to conduct a genome-wide identification and comprehensive characterization of the COBRA gene family members(CbuCOB)in Catalpa bungei,and to elucidate their transcriptional response patterns during tension wood(TW)formation.[Methods]Bioinformatic approaches were employed to identify and systematically analyze the CbuCOB genes.RNA sequencing was used to investigate the expression profiles of CbuCOB genes across different tissues and during TW formation in C.bungei.Quantitative real-time PCR(qRT-PCR)was further conducted to validate the expression levels of key CbuCOB genes.[Results]A total of 14 CbuCOB genes were identified in the C.bungei genome,exhibiting an uneven chromosomal distribution.Phylogenetic analysis classified these CbuCOB proteins into two subfamilies(SF I and SF II)and 12 subgroups,with members within the same subgroup exhibiting high structural similarity.Promoter cis-element analysis revealed that CbuCOB genes contain multiple elements associated with growth and development,environmental stress responses,and plant hormone signaling.Tissue-specific expression analysis indicated that CbuCOB genes exhibited distinct expression patterns in different tissues,with CbuCOB1,CbuCOB2,CbuCOB3,and CbuCOB9 showing higher expression levels in xylem.Temporal transcriptome analysis showed dynamic transcriptional responses of Cbu-COB genes during TW formation,with five SF I members(CbuCOB1,CbuCOB2,CbuCOB8,CbuCOB9,and CbuCOB12)and one SF II member(CbuCOB4)identified as differentially expressed genes.qRT-PCR validation confirmed that the expression trends of key CbuCOB genes were consistent with the transcriptome results,suggesting that these genes may be involved in regulating TW formation in C.bungei.[Conclusion]The COBRA gene family in C.bungei exhibits diverse gene characteristics and expression patterns,with several members likely playing key important roles in wood formation,particularly during TW formation.