文献类型：期刊文献

英文题名：Exogenous GA(3) application altered morphology, anatomic and transcriptional regulatory networks of hormones in Eucalyptus grandis

作者：Liu, Qian-Yu[1] Guo, Guang-Sheng[1] Qiu, Zhen-Fei[1] Li, Xiao-Dan[1] Zeng, Bing-Shan[1] Fan, Chun-Jie[1,2]

第一作者：Liu, Qian-Yu

通信作者：Zeng, BS[1];Fan, CJ[1];Fan, CJ[2]

机构：[1]Chinese Acad Forestry, Res Inst Trop Forestry, Guangzhou 510520, Guangdong, Peoples R China;[2]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China

年份：2018

卷号：255

期号：4

起止页码：1107-1119

外文期刊名：PROTOPLASMA

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000434795100011)】；

基金：This work was supported by National Natural Science Foundation of China (Grant No. 31400554) and the Fundamental Research Funds for the Central Non-profit Research Institution of CAF (Grant No. CAFYBB2014QB040).

语种：英文

外文关键词：Gibberellin; Eucalyptus grandis; Root; Xylem; Anatomical; Gene expression

摘要：Gibberellins (GAs) play a key role in plant growth and development including cell elongation, cell expansion, and xylem differentiation. Eucalyptus are the world's most widely planted hardwood trees providing fiber and energy. However, the roles of GAs in Eucalyptus remain unclear and their effects on xylem development remain to be determined. In this study, E. grandis plants were treated with 0.10 mg L-1 GA(3) and/or paclobutrazol (PAC, a GA inhibitor). The growth of shoot and root were recorded, transverse sections of roots and stems were stained using toluidine blue, and expression levels of genes related to hormone response and secondary cell wall biosynthesis were analyzed by quantitative real-time PCR. The results showed that GA(3) dramatically promoted the length of shoot and root, but decreased the diameter of root and stem. Exogenous GA(3) application also significantly promoted xylem development in both stem and root. Expression analysis revealed that exogenous GA(3) application altered the transcript levels of genes related to the GA biosynthetic pathway and GA signaling, as well as genes related to auxin, cytokinin, and secondary cell wall. These findings suggest that GAs may interact with other hormones (such as auxin and cytokinin) to regulate the expression of secondary cell wall biosynthesis genes and trigger xylogenesis in Eucalyptus plants.