文献类型：期刊文献

英文题名：Physiological and transcriptomic analysis provide novel insight into cobalt stress responses in willow

作者：Wang, Yi-Ming[1] Yang, Qi[2,3] Xu, Hui[3] Liu, Yan-Jing[3] Yang, Hai-Ling[1]

第一作者：Wang, Yi-Ming

通信作者：Yang, HL[1]

机构：[1]Beijing Forestry Univ, Coll Biol Sci & Biotechnol, Beijing 100083, Peoples R China;[2]Umea Univ, Umea Plant Sci Ctr, Dept Plant Physiol, SE-90187 Umea, Sweden;[3]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China

年份：2020

卷号：10

期号：1

外文期刊名：SCIENTIFIC REPORTS

收录：;Scopus(收录号：2-s2.0-85079334419);WOS:【SCI-EXPANDED(收录号:WOS:000529865500001)】；

基金：This study was supported by grant from the National Natural Science Foundation of China (NSFC 31570664).

语种：英文

摘要：Cobalt (Co) is an essential component of several enzymes and coenzymes in living organisms. Excess Co is highly toxic to plants. The knowledge of molecular response mechanisms to cobalt stress in plants is still limited, especially in woody plants. The responses of weeping willow (Salix babylonica) seedlings to Co stress were studied using morphological and physiochemical measurements and RNA-seq analysis. The physiological and biochemical indexes such as growth rate, the content of chlorophyll and soluble sugar, photosynthesis and peroxidase activity were all changed in willow seedlings under Co stress. The metal ion concentrations in willow including Cu, Zn and Mg were disturbed due to excess Co. Of 2002 differentially expressed genes (DEGs), 1165 were root-specific DEGs and 837 were stem and leaf-specific DEGs. Further analysis of DEGs showed there were multiple complex cascades in the response network at the transcriptome level under Co stress. Detailed elucidation of responses to oxidative stress, phytohormone signaling-related genes and transcription factors (TFs), and detoxification of excess cellular Co ion indicated the various defense mechanisms in plants respond to cobalt stress. Our findings provide new and comprehensive insights into the plant tolerance to excess Co stress.