文献类型：期刊文献

英文题名：Proteomic Analysis and Identification of Possible Allergenic Proteins in Mature Pollen of Populus tomentosa

作者：Wang, Liuqiang[1,2] Zhang, Xiaoling[1] Zhang, Jin[1] Fan, Wei[1] Lu, Mengzhu[1,2] Hu, Jianjun[1,2]

第一作者：王留强;Wang, Liuqiang

通信作者：Hu, JJ[1];Hu, JJ[2]

机构：[1]Chinese Acad Forestry, State Forestry Adm, Key Lab Tree Breeding & Cultivat, Res Inst Forestry,State Key Lab Tree Genet & Bree, Beijing 100091, Peoples R China;[2]Nanjing Forestry Univ, Coinnovat Ctr Sustainable Forestry Southern Chin, Nanjing 210037, Peoples R China

年份：2018

卷号：19

期号：1

外文期刊名：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000424407200245)】；

基金：This work was supported by the National Nonprofit Institute Research Grant of the Chinese Academy of Forestry (CAFYBB2017ZY008) and Special Fund for Forestry Scientific Research in the Public Interest (201304103-4). We also thank the editor and reviewers for critical comments and thoughtful suggestions.

语种：英文

外文关键词：allergen; MALDI-TOF; TOFMS; MS; pollen; Populus tomentosa; two-dimensional gel electrophoresis

摘要：Pollen grains from Populus tomentosa, a widely cultivated tree in northern area of China, are considered to be an important aeroallergen causing severe allergic diseases. To gain insight into their allergenic components, mature Populus tomentosa pollen proteins were analyzed by two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF/TOF MS). A total of 412 spots from mature pollen were resolved on pH 4-7 immobilized pH gradient (IPG) strips and 159 distinct proteins were identified from 242 spots analyzed. The identified proteins were categorized based on their functional role in the pollen, which included proteins involved in energy regulation, protein fate, protein synthesis and processing, metabolism, defense/stress responses, development and other functional categories. Moreover, among the identified proteins, 27 proteins were identified as putative allergens using the Structural Database of Allergenic Proteins (SDAP) tool and Allergen Online. The expression patterns of these putative allergen genes indicate that several of these genes are highly expressed in pollen. The identified putative allergens have the potential to improve specific diagnosis and can be used to develop vaccines for immunotherapy against poplar pollen allergy.