文献类型：期刊文献

英文题名：Homolog of Human placenta-specific gene 8, PcPLAC8-10, enhances cadmium uptake by Populus roots

作者：Cheng, Yao[1] Chen, Xin[1] Liu, Wenzhe[1] Yang, Lingyu[1] Wu, Jiangting[1] Wang, Yang[1] Yu, Wenjian[1] Zhou, Jing[1] Fayyaz, Payam[2] Luo, Zhi-Bin[1,3,4] Deng, Shurong[1] Shi, Wenguang[1]

第一作者：Cheng, Yao

机构：[1] National Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, China; [2] Forest, Range and Watershed Management Department, Agriculture and Natural Resources Faculty, Yasouj University, Yasuj, 75919 63179, Iran; [3] Institute of Ecological Protection and Restoration, Chinese Academy of Forestry, Beijing, 100091, China; [4] Comprehensive Experimental Center of Chinese Academy of Forestry in Yellow River Delta, Shandong Province, Dongying, 257000, China

年份：2023

卷号：460

外文期刊名：Journal of Hazardous Materials

收录：EI(收录号：20233514659306);Scopus(收录号：2-s2.0-85168995921)

语种：英文

外文关键词：Bioremediation - Cadmium - Cell membranes - Plants (botany) - Soil pollution

摘要：Cadmium (Cd) pollution of soil occurs worldwide. Phytoremediation is an effective approach for cleaning up Cd polluted soil. Fast growing Populus species with high Cd uptake capacities are desirable for phytoremediation. Thus, it is important to elucidate the molecular functions of genes involved in Cd uptake by poplars. In this study, PcPLAC8–10, a homolog of Human placenta-specific gene 8 (PLAC8) implicated in Cd transport was functionally characterized in Populus × canescens. PcPLAC8–10 was transcriptionally induced in Cd-treated roots and it encoded a plasma membrane-localized transporter. PcPLAC8–10 exhibited Cd uptake activity when expressed in yeast cells. No difference in growth was observed between wild type (WT) and PcPLAC8–10-overexpressing poplars. PcPLAC8–10-overexpressing poplars exhibited increases in net Cd2+ influxes by 192% and Cd accumulation by 57% in the roots. However, similar reductions in biomass were found in WT and transgenic poplars when exposed to Cd. The complete motif of CCXXXXCPC in PcPLAC8–10 was essential for its Cd transport activity. These results suggest that PcPLAC8–10 is a plasma membrane-localized transporter responsible for Cd uptake in the roots and the complete CCXXXXCPC motif of PcPLAC8–10 plays a key role in its Cd transport activity in poplars. ? 2023 Elsevier B.V.