文献类型：期刊文献

英文题名：Effect of Alkali Lignin on Laccase Activity and Mycelial Biomass of Flammulina velutipes

作者：Han, Mei-Ling[1,2,3] Zhao, Yi-Lin[1] Liu, Tian-Yu[1] Bian, Lu-Sen[4] Li, Zi-Han[1] Li, Ming-Xue[1] Li, Jia[1] Yang, Xiao-Qi[1] An, Qi[1,2]

第一作者：Han, Mei-Ling

通信作者：An, Q[1];An, Q[2]

机构：[1]Langfang Normal Univ, Coll Life Sci, Langfang 065000, Hebei, Peoples R China;[2]Tech Innovat Ctr Utilizat Edible & Med Fungi Hebei, Langfang 065000, Hebei, Peoples R China;[3]Edible & Med Fungi Res & Dev Ctr Univ Coll Hebei P, Langfang 065000, Hebei, Peoples R China;[4]Chinese Acad Forestry, Expt Ctr Forestry North China, Warm Temperate Zone Forestry Jiulong Mt Natl Perma, Beijing 102300, Peoples R China

年份：2025

卷号：20

期号：1

起止页码：2243-2258

外文期刊名：BIORESOURCES

收录：;EI(收录号：20250717857089);Scopus(收录号：2-s2.0-85217279808);WOS:【SCI-EXPANDED(收录号:WOS:001452828700033)】；

基金：This research was supported by the Fundamental Research Funds for the Central Non-profit Research Institution of the Chinese Academy of Forestry (Project No. CAFYBB2021MA007) , the Hebei Natural Science Foundation (C2024408007) , the Fundamental Research Funds for the Universities in Hebei Province (JYQ202201) , and the Top-notch Youth Project of Langfang City, China.

语种：英文

外文关键词：Flammulina velutipes; Laccase; Alkali lignin; Populus beijingensis; Mycelial biomass

摘要：The effect of alkali lignin on laccase activity and mycelial biomass of Flammulina velutipes was investigated at different fermentation temperatures. The secretion of laccase by F. velutipes has certain specificity. Under most fermentation medium conditions, no laccase activity of F. velutipes CCMSSC 00103 was detected, but laccase activity of F. velutipes FL 19 could be detected. Among the three strains of F. velutipes, the laccase secretion capacity of F. velutipes FL 19 was clearly higher than that of F. velutipes CCMSSC 05331 and F. velutipes CCMSSC 00103. Maximum laccase activity with the value of 73.42 +/- 4.74 U/L was secreted by F. velutipes FL 19 in fermentation medium with alkali lignin, KH2PO4, Vitamin B1, and glucose, and appeared on the 2nd day. The presence of alkali lignin was useful for improving laccase activity secreted by F. velutipes. The effect of alkali lignin on improving laccase activity exceeded that of Populus beijingensis. F. velutipes was more suitable for secreting laccase at 26 degrees C because the maximum laccase activity at 26 degrees C (73.42 +/- 4.74 U/L) was higher than that measured at 33 degrees C (70.71 +/- 6.89 U/L). The results can be useful for selecting suitable medium and temperature of F. velutipes strains to produce low-cost laccase.