文献类型：期刊文献

英文题名：Ultrasound-assisted deep eutectic solvents extraction of polysaccharides from Loquat leaf: Process optimization and bioactivity study

作者：Yan, Ke[1] Liu, Xianglin[1] Li, Lin[1] Zhu, Shuyu[1] Zheng, Lijuan[3] He, Shuyang[1] Jia, Xiaomin[4] Dong, Wuzi[5] Liu, Yupeng[6] Lu, Zhoumin[1] Yang, Fangxia[1,2]

第一作者：Yan, Ke

通信作者：Lu, ZM[1];Yang, FX[1];Yang, FX[2]

机构：[1]Northwest A&F Univ, Coll Forestry, Yangling 712100, Shaanxi, Peoples R China;[2]Northwest A&F Univ, Coll Forestry, Shaanxi Prov Key Lab Econ Plant Resources Dev & Ut, Yangling 712100, Shaanxi, Peoples R China;[3]Northwest A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Shaanxi, Peoples R China;[4]Yanan Univ, Sch Life Sci, Yanan 716000, Shaanxi, Peoples R China;[5]Shaanxi Dayi Xunlong Biotechnol Co LTD, Yangling 712100, Shaanxi, Peoples R China;[6]Chinese Acad Forestry, Inst Chem Ind Forest Prod, Natl Engn & Technol Res Ctr Forest Chem Ind, Nanjing 210037, Jiangsu, Peoples R China

年份：2024

卷号：274

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

收录：;EI(收录号：20242616422153);Scopus(收录号：2-s2.0-85196796465);WOS:【SCI-EXPANDED(收录号:WOS:001263465000001)】；

基金：This work was supported by the project of Shaanxi Academy of Forestry Science [SKLK2023-02-27] .

语种：英文

外文关键词：Deep eutectic solvents; Ultrasonic-assisted extraction; Polysaccharides; Semen preservation

摘要：Loquat leaves are the by-product of loquat fruit production. Polysaccharides are one of the main active ingredients in loquat leaves. In this study, polysaccharides were extracted from loquat leaves by ultrasonic-assisted deep eutectic solvents (DESs) extraction method. Further, the extracted crude loquat leaf polysaccharides (CLLP) were purified and separated via S-8 resin and DEAE-52 cellulose column chromatography, respectively. Additionally, the effects of polysaccharides on activity of sperm in boar semen preserved in medium at 17 degrees C, were evaluated preliminarily. DES, composed of choline chloride/ethylene glycol (1:6, molar ratio), was proved to be the suitable solvent for LLP extraction. The optimized extraction conditions were water content 44 %, liquid-solid ratio 1:29 (g/g), extraction temperature 61 degrees C and extraction time 98 min. Under these conditions, the LLP yield was 57.82 +/- 1.50 mg/g. A homogeneous polysaccharide (LLP1-2, M w : 2.17 x 10 4 Da) was isolated from CLLP. Its total sugar, uronic acid and protein contents were 76.31 +/- 1.25 %, 14.19 +/- 0.67 % and 3.28 +/- 0.42 %, respectively. Further, 800 mu g/mL LLP1-2 could effectively enhance the antioxidant activity of sperm. This study laid a foundation for DESs and column chromatography in the field of polysaccharide extraction and separation, proving that LLP can be used as a natural antioxidant for sperm preservation.