文献类型：期刊文献

英文题名：Comprehensive identification and expression analyses of the SnRK gene family in Casuarina equisetifolia in response to salt stress

作者：Ai, Di[1,2] Wang, Yujiao[1] Wei, Yongcheng[1] Zhang, Jie[2] Meng, Jingxiang[1] Zhang, Yong[1]

第一作者：Ai, Di

通信作者：Zhang, Y[1]

机构：[1]Res Inst Trop Forestry, Chinese Acad Forestry, Guangzhou 510520, Peoples R China;[2]Northeast Forestry Univ, Coll Landscape Architecture, Harbin, Peoples R China

年份：2022

卷号：22

期号：1

外文期刊名：BMC PLANT BIOLOGY

收录：;Scopus(收录号：2-s2.0-85143559750);WOS:【SCI-EXPANDED(收录号:WOS:000895906300001)】；

基金：This study was financially supported by the Open Project of Key Laboratory of Ministry of Education on Tropical Island Ecology (HNSF-OP-202001), the National Natural Science Foundation of China (Grant No. 31901334) and the 7th Scientific and Technological Research project of Forest Seedlings in Fujian province (FJZM 2021-03). The funding bodies were not involved in the design of the study and collection, analysis, and interpretation of data and in writing the manuscript.

语种：英文

外文关键词：Phylogenetic analysis; Cis-regulatory element analysis; Expression pattern; Salt stress

摘要：Background: Sucrose nonfermenting-1 (SNF1)-related protein kinases (SnRKs) play crucial roles in plant signaling pathways and stress adaptive responses by activating protein phosphorylation pathways. However, there have been no comprehensive studies of the SnRK gene family in the widely planted salt-tolerant tree species Casuarina equisetifolia. Here, we comprehensively analyze this gene family in C. equisetifolia using genome-wide identification, characterization, and profiling of expression changes in response to salt stress. Results: A total of 26 CeqSnRK genes were identified, which were divided into three subfamilies (SnRK1, SnRK2, and SnRK3). The intron-exon structures and protein-motif compositions were similar within each subgroup but differed among groups. Ka/Ks ratio analysis indicated that the CeqSnRK family has undergone purifying selection, and cis-regulatory element analysis suggested that these genes may be involved in plant development and responses to various environmental stresses. A heat map was generated using quantitative real-time PCR (RT-qPCR) data from 26 CeqSnRK genes, suggesting that they were expressed in different tissues. We also examined the expression of all CeqSnRK genes under exposure to different salt concentrations using RT-qPCR, finding that most CeqSnRK genes were regulated by different salt treatments. Moreover, co-expression network analysis revealed synergistic effects among CeqSnRK genes. Conclusions: Several CeqSnRK genes (CeqSnRK3.7, CeqSnRK3.16, CeqSnRK3.17) were up-regulated following salt treatment. Among them, CeqSnRK3.16 expression was significantly up-regulated under various salt treatments, identifying this as a candidate gene salt stress tolerance gene. In addition, CeqSnRK3.16 showed significant expression change correlations with multiple genes under salt stress, indicating that it might exhibit synergistic effects with other genes in response to salt stress. This comprehensive analysis will provide a theoretical reference for CeqSnRK gene functional verification and the role of these genes in salt tolerance.