文献类型：期刊文献

英文题名：Isolation, characterization and phylogenetic analysis of nucleotide binding site-encoding disease-resistance gene analogues from European Aspen (populus tremula)

作者：Zhang, Yong[1,2,4] Zhang, Shougong[3] Qi, Liwang[3] Zhang, Tao[1] Wang, Chunguo[2] Chen, Chengbin[2] Song, Wenqin[2,5]

第一作者：Zhang, Yong

通信作者：Song, W.

机构：[1] School of Life Sciences and Technology, University of Electronic Science and Technology of China, Chendu 610054, China; [2] College of Life Sciences, Nankai University, Tianjin 300071, China; [3] Laboratory of Cell Biology, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China; [4] Laboratory of Molecular Cytogenetic Biology, School of Life Sciences and Technology, University of Electronic Science and Technology of China, Chendu, 610054, China; [5] Laboratory of Cell Biology, College of Life Sciences, Nankai University, Tianjin 300071, China

年份：2010

卷号：59

期号：2-3

起止页码：68-77

外文期刊名：Silvae Genetica

收录：EI(收录号：20103313160305);Scopus(收录号：2-s2.0-77955605963)

语种：英文

外文关键词：Signal encoding - Amino acids - Cloning - Plants (botany) - Forestry - Nucleotides - Gene encoding

摘要：The majority of verified plant disease resistance genes (R genes) isolated to date was of the nucleotide binding site-leucine rich repeat (NBS-LRR) class. The conservation between different NBS-LRR R genes opens the avenue for the use of PCR based strategies in isolating and cloning other R gene family members or analogs (resistance gene analogue, RGA) using degenerate primers for these conserved regions. In this study, to better understand the R gene in European aspen (Populus tremula), a perennial tree, we used degenerate primers to amplify RGA sequences from European aspen. Cloning and sequence characterization identified 37 European aspen RGAs, which could be phylogenetically classified into seven subfamilies. Deduced amino acid sequences of European aspen RGAs showed strong identity, ranging from 30.41 to 46.63%, to toll interleukin receptor (TIR) R gene subfamily. BLAST searches with reference to the genomic sequence of P. trichocarpa found 209 highly homologous regions distributed in 28 genomic loci, suggesting the abundance and divergence of NBS-encoding R genes in European aspen genome. Although, numerous studies have reported that plant R genes are under diversifying selection for specificity to evolving pathogens, non-synonymous to synonymous nucleotide substitution (dN/d s) ratio were