文献类型：期刊文献

英文题名：Isolation and Characterization of Three Chalcone Synthase Genes in Pecan (Carya illinoinensis)

作者：Zhang, Chengcai[1] Yao, Xiaohua[1] Ren, Huadong[1] Wang, Kailiang[1] Chang, Jun[1]

第一作者：张成才

通信作者：Yao, XH[1]|[a00051e90d3009f1c7c75]姚小华;

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou 311400, Zhejiang, Peoples R China

年份：2019

卷号：9

期号：6

外文期刊名：BIOMOLECULES

收录：;Scopus(收录号：2-s2.0-85068495959);WOS:【SCI-EXPANDED(收录号:WOS:000475301500032)】；

基金：This research was funded by Fundamental Research Funds of CAF No. CAFYBB2018SY013, and the National Natural Science Foundation of China No. 31800575.

语种：英文

外文关键词：pecan; Carya illinoinensis; phenolics; flavonoid; chalcone synthase; CHS; correlation analysis

摘要：Phenolics are a group of important plant secondary metabolites that have been proven to possess remarkable antioxidant activity and to be beneficial for human health. Pecan nuts are an excellent source of dietary phenolics. In recent years, many studies have focused on the separation and biochemical analysis of pecan phenolics, but the molecular mechanisms of phenolic metabolism in pecans have not been fully elucidated, which significantly hinders quality breeding research for this plant. Chalcone synthase (CHS) plays crucial roles in phenolic biosynthesis. In this study, three Carya illinoinensis CHSs (CiCHS1, CiCHS2, and CiCHS3), were isolated and analyzed. CiCHS2 and CiCHS3 present high expression levels in different tissues, and they are also highly expressed at the initial developmental stages of kernels in three pecan genotypes. A correlation analysis was performed between the phenolic content and CHSs expression values during kernel development. The results indicated that the expression variations of CiCHS2 and CiCHS3 are significantly related to changes in total phenolic content. Therefore, CiCHSs play crucial roles in phenolic components synthesis in pecan. We believe that the isolation of CiCHSs is helpful for understanding phenolic metabolism in C. illinoinensis, which will improve quality breeding and resistance breeding studies in this plant.