文献类型：期刊文献

中文题名：美国白蛾核型多角体病毒多角体蛋白的SDS-PAGE分析

英文题名：SDS-PAGE Analysis on Protein of Hyphantria cunea Nuclear Polyhedrosis Virus

作者：薛建杰[1,2] 曲良建[3] 王玉珠[3] 张永安[3] 唐明[1] 杨唯一[1,2]

第一作者：薛建杰

机构：[1]西北农林科技大学林学院;[2]中国林业科学研究院森林生态环境与保护研究所,国家林业局森林保护学重点实验室;[3]中国林业科学研究院森林生态环境与保护研究所国家林业局森林保护学重点实验室

年份：2009

卷号：22

期号：5

起止页码：728-731

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金：国家自然科学基金(30671688)资助;国家“十一五”科技支撑计划(2006BAD08A1202)

语种：中文

中文关键词：HcNPV;核型多角体病毒;多角体蛋白;SDS-PAGE;32KD蛋白

外文关键词：nuclear polyhedrosis ; polyhedrin ; SDS-PAGE ; 32 KD protein

分类号：S476.13;S763.42

摘要：Hyphantria cuea nucleopolyhedrovirus(HcNPV) and several other nucleopolyhedrosis was extracted by differential centrifugation,and purified with sucrose gradient centrifugation method.Polyhedrin was gotten and analysed by SDS-PAGE.It indicated that most of those proteins had 32 KD protein band,and with 64 KD protein,and might be the main protein dimer with the structure and found Hyphantria cunea nuclear polyhedrosis virus protein in 18 KD department had a specific band.
Hyphantria cuea nucleopolyhedrovirus （HcNPV） and several other nucleopolyhedrosis was extracted by differential centrifugation, and purified with sucrose gradient centrifugation method. Polyhedrin was gotten and analysed by SDS - PAGE. It indicated that most of those proteins had 32 KD protein band, and with 64 KD protein, and might be the main protein dimer with the structure and found Hyphantria cunea nuclear polyhedrosis virus protein in 18 KD department had a specific band.