文献类型：期刊文献

英文题名：Hybrid-Transcriptome Sequencing and Associated Metabolite Analysis Reveal Putative Genes Involved in Flower Color Difference in Rose Mutants

作者：Huang, Ping[1] Lin, Furong[1] Li, Bin[1] Zheng, Yongqi[1]

第一作者：黄平

通信作者：Huang, P[1];Zheng, YQ[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Lab Forest Silviculture & Tree Cultivat, Beijing 100091, Peoples R China

年份：2019

卷号：8

期号：8

外文期刊名：PLANTS-BASEL

收录：;Scopus(收录号：2-s2.0-85070962646);WOS:【SCI-EXPANDED(收录号:WOS:000483537400005)】；

基金：This research was supported by Fundamental Research Funds for the Central Non-profit Research Institution of CAF, grant number CAFYBB2016QB007, and National Natural Science Foundation of China, grant number 31500538.

语种：英文

外文关键词：Rosa; flower color variation; flavonoid biosynthesis; transcriptome; metabolite analysis

摘要：Gene mutation is a common phenomenon in nature that often leads to phenotype differences, such as the variations in flower color that frequently occur in roses. With the aim of revealing the genomic information and inner mechanisms, the differences in the levels of both transcription and secondary metabolism between a pair of natural rose mutants were investigated by using hybrid RNA-sequencing and metabolite analysis. Metabolite analysis showed that glycosylated derivatives of pelargonidin, e.g., pelargonidin 3,5 diglucoside and pelargonidin 3-glucoside, which were not detected in white flowers (Rosa 'Whilte Mrago Koster'), constituted the major pigments in pink flowers. Conversely, the flavonol contents of petal, such as kaempferol-3-glucoside, quercetin 3-glucoside, and rutin, were higher in white flowers. Hybrid RNA-sequencing obtained a total of 107,280 full-length transcripts in rose petal which were annotated in major databases. Differentially expressed gene (DEG) analysis showed that the expression of genes involved in the flavonoid biosynthesis pathway was significantly different, e.g., CHS, FLS, DFR, LDOX, which was verified by qRT-PCR during flowering. Additionally, two MYB transcription factors were found and named RmMYBAN2 and RmMYBPA1, and their expression patterns during flowering were also analyzed. These findings indicate that these genes may be involved in the flower color difference in the rose mutants, and competition between anthocyanin and flavonol biosynthesis is a primary cause of flower color variation, with its regulation reflected by transcriptional and secondary metabolite levels.