文献类型：期刊文献

中文题名：印度黄檀茎段腋芽诱导培养研究

英文题名：Study on Axillary Buds Induction from Stem Segments of Dalbergia sissoo Roxb

作者：杜婷[1] 牛慈琼[2] 石雷[1] 廖怀建[1]

第一作者：杜婷

机构：[1]中国林业科学研究院资源昆虫研究所;[2]山西省农业科学院果树研究所

年份：2016

卷号：29

期号：12

起止页码：2959-2964

中文期刊名：西南农业学报

外文期刊名：Southwest China Journal of Agricultural Sciences

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD_E2015_2016】；

基金：国家科技支撑计划项目(2012BAD01B05);国家重点研发计划项目(2016YFD06006005)

语种：中文

中文关键词：印度黄檀;消毒;腋芽;诱导培养

外文关键词：Dalbergia sissoo Roxb; Sterilization methods; Basal medium; Axillary buds induction; Vitrification

分类号：S772.37

摘要：为建立有效的印度黄檀组培快繁技术，本研究采用优树嫁接苗的腋芽茎段作为外植体，阐明不同消毒方法、不同基础培养基、不同植物生长调节剂和活性炭浓度对诱导培养的影响，以期为获得能够保持母株优良性状的无性系苗木奠定基础。结果表明，最佳的消毒方法是，外植体先用75％酒精浸泡30s，然后用0．1％升汞浸泡10min，此时污染率最低，腋芽萌发率最高，污染率和存活率分别为（16．67±2．03）％和（70．33±2．03）％；相对于B5和WPM基础培养基，使用MS培养基对外植体进行培养，萌芽率和苗高最高，分别为（85．00±2．89）％和（22．03±0．09）mm；培养基中植物生长调节剂6-BA和NAA的浓度及两者浓度比率均对腋芽诱导产生显著影响；当NAA浓度（0．01～0．02mg／L）较低时，在0．2～0．3mg／L范围内提高6-BA的浓度有利于植株生长，且两者浓度比率达到20～30时，最适宜腋芽诱导；在最适激素浓度条件下，添加0．5∥L活性炭，能够有效提高外植体萌芽率，降低玻璃化率。综上所述，印度黄檀茎段腋芽诱导的最适培养基为MS＋0．3mg／L6-BA＋0．01mg／LNAA＋0．5g／L。
In order to establish effective rapid propagation technique for Dalbergia sissoo Roxb, axillary buds from stem segments of selected tree grafted seedlings were used as explants in this paper. Effects of different disinfection methods, different basic medium component, and different concentration of plant growth regulator and the activated carbon on its germination of axillary buds were studied for attaining clone seedlings maintained mother plants＇ excellent characters. The results showed that the time of 75 % alcohol disinfection was 30 seconds and the time of 0.1% HgC12 disinfection was 10 minutes, in this treatment, the lowest pollution rate was （ 16.67 ± 2.03 ） % and the highest germination rate of the bud was （70.33 ± 2.03 ） %. Compared with Bsand WPM, MS was the optimal culture medium with the highest germination rate （ 85.00 ± 2.89 ） % and seedling height（ 22.03 mm± 0.09 mm）. The concentration and proportion of 6-BA and NAA had the influence on the explants bud induction. When the concentration of NAA was low （0.01 -0.02 mg/L） , increasing 6-BA concentration in the range from 0. 2 to 0.3 mg/L was beneficial to plant growth. And the proportion of 6-BA and NAA in the range from 20 to 30 was advantageous to the axillary bud induction. Under the condition of the optimal concentration of plant growth regulator, adding 0. 5 g/L activated carbon in the medium could effectively improve the germination rate of explants and reduce the rate of vitrification. In conclusion, this study suggested that the medium composition of MS ＋ 0.3 mg/L 6-BA ＋ 0. 01 mg/L NAA ＋ 0.5 g/L was the optimum for axillary buds induction from stem segments of Dalbergia sissoo Roxb.