文献类型：期刊文献

英文题名：Integrating Iso-seq and RNA-seq analyses to unveil bidirectional movement of mRNAs in oil-Camellia heterografting

作者：Ge, Xiaoning[1,2] Tan, Xinjian[3] Cao, Linqing[1] Zhong, Qiuping[1] Chen, Chuansong[1] Wang, Jinfeng[1] Zhou, Youcheng[1] Zou, Yuling[1]

第一作者：葛晓宁

通信作者：Cao, LQ[1];Tan, XJ[2]

机构：[1]Chinese Acad Forestry, Expt Ctr Subtrop Forestry, Fenyi 336600, Jiangxi, Peoples R China;[2]Chinese Acad Forestry, Inst Forest Resource Informat Tech, Beijing 100091, Peoples R China;[3]Chinese Acad Forestry, Beijing 100091, Peoples R China

年份：2026

卷号：241

外文期刊名：INDUSTRIAL CROPS AND PRODUCTS

收录：;EI(收录号：20260319936851);Scopus(收录号：2-s2.0-105027637917);WOS:【SCI-EXPANDED(收录号:WOS:001674906100001)】；

基金：We are grateful to the Chinese Academy of Forestry, Experimental Center of Subtropical Forestry, for funding and field support, and to the Central South University of Forestry and Technology for laboratory and theoretical knowledge support. This study was funded by the Funda-mental Research Fund of CAF (CAFYBB2022MA008) .

语种：英文

外文关键词：Heterografting; mRNA movement; Genetic transformation; Iso-seq; Camellia oleifera

摘要：Grafting has long been the primary technical method for breeding oil-Camellia varieties and improving crop performance. This is a major factor limiting Camellia oil yield. mRNAs are key signaling molecules involved in stock-scion interactions. Although grafting is traditionally considered a form of asexual reproduction, it can induce phenotypic variations that are potentially driven by genetic material transfer and epigenetic regulation. However, little is known about the role of mRNA movement between rootstocks and scions in heterografting. In this study, we investigated mobile mRNAs involved in heterografting between Camellia oleifera and Camellia chekiangoleosa using third-generation full-length transcriptome sequencing (Iso-seq) and second-generation transcriptome sequencing (RNA-seq) analyses. Bidirectional movement of mRNAs was observed after grafting, and these mobile mRNAs were significantly enriched in pathways related to plant hormone signal transduction and auxin biosynthesis. To validate the mRNA movement events, heterografting experiments were conducted between transgenic Xiangyan No. 7 (harboring the key genes GH3 and UGT involved in hormone signaling and auxin biosynthesis) and wild-type Nicotiana x sanderae. The results confirmed bidirectional mRNA movement, with the transferred mRNAs remaining functional in the recipient cells. These findings elucidate the critical mechanisms of rootstock-scion interaction in oil-Camellia heterografting, highlighting mRNA mobility as a regulatory factor in genetic information exchange. This study advances our understanding of grafting biology and provides a foundation for novel grafting-based strategies to enhance crop breeding and yield.